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Literature summary for 2.4.1.7 extracted from
- Molecular cloning of a gene encoding the sucrose phosphorylase from Leuconostoc mesenteroides B-1149 and the expression in Escherichia coli (2006), Enzyme Microb. Technol., 39, 612-620.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Leuconostoc mesenteroides |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.3 | - |
sucrose | - |
Leuconostoc mesenteroides |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56100 | - |
x * 56100, calculation from sequence | Leuconostoc mesenteroides |
| 60000 | - |
x * 60000, SDS-PAGE | Leuconostoc mesenteroides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leuconostoc mesenteroides | Q14EH6 | - |
- |
| Leuconostoc mesenteroides B-1149 | Q14EH6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Leuconostoc mesenteroides |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.49 | - |
- |
Leuconostoc mesenteroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + acarbose | transglucosylation | Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + cellobiose | transglucosylation | Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + cellobiose | transglucosylation | Leuconostoc mesenteroides B-1149 | ? | - |
? | |
| sucrose + D-arabinose | transglucosylation | Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + D-arabinose | transglucosylation | Leuconostoc mesenteroides B-1149 | ? | - |
? | |
| sucrose + D-fructose | transglucosylation | Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + D-glucose | transglucosylation | Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + erythritol | transglucosylation | Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + galactose | transglucosylation | Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + isomaltotriose | transglucosylation | Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + lactose | transglucosylation | Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + lactose | transglucosylation | Leuconostoc mesenteroides B-1149 | ? | - |
? | |
| sucrose + maltose | transglucosylation | Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + maltose | transglucosylation | Leuconostoc mesenteroides B-1149 | ? | - |
? | |
| sucrose + maltotriose | transglucosylation | Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + maltotriose | transglucosylation | Leuconostoc mesenteroides B-1149 | ? | - |
? | |
| sucrose + palatinose | transglucosylation | Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + rhamnose | transglucosylation | Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + salicin | transglucosylation | Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + sorbitol | transglucosylation | Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + xylitol | transglucosylation | Leuconostoc mesenteroides | ? | - |
? | |
| sucrose + xylose | transglucosylation | Leuconostoc mesenteroides | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, SDS-PAGE | Leuconostoc mesenteroides |
| ? | x * 56100, calculation from sequence | Leuconostoc mesenteroides |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1149SPase | - |
Leuconostoc mesenteroides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | - |
Leuconostoc mesenteroides |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 75 | 20°C-50°C: maximal activity, 75°C: about 50% of maximal activity | Leuconostoc mesenteroides |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
30 min, enzyme retains over 70% of the initial activity | Leuconostoc mesenteroides |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.59 | - |
sucrose | - |
Leuconostoc mesenteroides | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | - |
Leuconostoc mesenteroides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | pH 5.0: about 40% of maximal activity, pH 8.0: about 20% of maximal activity | Leuconostoc mesenteroides |
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