Any feedback?
Literature summary for 2.4.1.7 extracted from
- Mechanism of action of sucrose phosphorylase. III. The reaction with water and other alcohols (1972), J. Biol. Chem., 247, 532-542.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Pelomonas saccharophila |  |
| Chloroethanol | - |
Pelomonas saccharophila | |
| ethylene glycol | noncompetitive with sucrose and phosphate | Pelomonas saccharophila | |
| trans-1,2-cyclohexanediol | noncompetitive with sucrose, competitive with phosphate | Pelomonas saccharophila | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
phosphate | - |
Pelomonas saccharophila | |
| 13 | - |
fructose | - |
Pelomonas saccharophila | |
| 130 | - |
L-sorbose | - |
Pelomonas saccharophila | |
| 390 | - |
cis-1,2-Cyclohexanediol | - |
Pelomonas saccharophila | |
| 400 | - |
trans-1,2-cyclohexanediol | - |
Pelomonas saccharophila | |
| 870 | - |
ethylene glycol | - |
Pelomonas saccharophila | |
| 2100 | - |
ethanol | - |
Pelomonas saccharophila | |
| 2300 | - |
methanol | - |
Pelomonas saccharophila | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + phosphate | Pelomonas saccharophila | - |
alpha-D-glucose 1-phosphate + D-fructose | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| acetonitrile | inactivation of enzyme | Pelomonas saccharophila |
| dioxane | inactivation of enzyme | Pelomonas saccharophila |
| Ethylene glycol | stable up to 30% | Pelomonas saccharophila |
| Methanol | stable up to 30% | Pelomonas saccharophila |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pelomonas saccharophila | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate + cis-1,2-cyclohexanediol | - |
Pelomonas saccharophila | hydroxycyclohexylglucoside + phosphate | - |
? | |
| alpha-D-glucose 1-phosphate + ethanol | low glycosyl-acceptor efficiency | Pelomonas saccharophila | alpha-D-ethylglucoside + phosphate | - |
? | |
| alpha-D-glucose 1-phosphate + ethylene glycol | - |
Pelomonas saccharophila | alpha-hydroxyethyl-D-glucoside + phosphate | - |
? | |
| alpha-D-glucose 1-phosphate + H2O | - |
Pelomonas saccharophila | alpha-D-glucose + phosphate | - |
ir | |
| alpha-D-glucose 1-phosphate + methanol | - |
Pelomonas saccharophila | alpha-D-methylglucoside + phosphate | - |
? | |
| alpha-D-glucose 1-phosphate + trans-1,2-cyclohexanediol | - |
Pelomonas saccharophila | hydroxycyclohexylglucoside + phosphate | - |
? | |
| alpha-D-glucose-1-phosphate + L-sorbose | - |
Pelomonas saccharophila | alpha-D-glucosyl-alpha-L-sorbose + phosphate | - |
? | |
| L-sorbose + alpha-D-glucose-1-phosphate | - |
Pelomonas saccharophila | alpha-D-glucopyranosyl-(1->2)-beta-L-sorbose + phosphate | - |
? | |
| additional information | sucrose and alpha-glucose-1-phosphate are hydrolyzed in absence of phosphate and arsenate at very low rate | Pelomonas saccharophila | ? | - |
? | |
| sucrose + cis-1,2-cyclohexanediol | - |
Pelomonas saccharophila | ? | - |
? | |
| sucrose + ethanol | - |
Pelomonas saccharophila | alpha-D-glucose + beta-D-fructose + alpha-D-ethylglucoside | - |
? | |
| sucrose + ethylene glycol | - |
Pelomonas saccharophila | ? | - |
? | |
| sucrose + methanol | - |
Pelomonas saccharophila | alpha-D-glucose + beta-D-fructose + alpha-D-methylglucoside | - |
? | |
| sucrose + phosphate | - |
Pelomonas saccharophila | alpha-D-glucose 1-phosphate + D-fructose | - |
? | |
| sucrose + phosphate | - |
Pelomonas saccharophila | alpha-D-glucose 1-phosphate + D-fructose | - |
r | |
| sucrose + trans-1,2-cyclohexanediol | - |
Pelomonas saccharophila | ? | - |
? | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 250 | - |
trans-1,2-cyclohexanediol | - |
Pelomonas saccharophila | |
| 2500 | - |
ethylene glycol | - |
Pelomonas saccharophila | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
