Protein Variants | Comment | Organism |
---|---|---|
additional information | 293/EGFR/FUT8, HEK-293 cells transfected with epidermal growth factor receptor and FUT8, increased fucosylation of EGFR, about 20% increase of cellular growth in response to epidermal growth factor stiumlation, enhanced cellular sensitivity to gefitinib, decrease of epidermal growth factor-mediated phosphorylation of mitogen-activated protein kinase | Homo sapiens |
additional information | 293/EGFR/shFUT8, stable knockdown mutant of intrinsic FUT8, about 20% decrease of cellular growth in response to epidermal growth factor stimulation | Homo sapiens |
additional information | A549/FUT8, overexpression of FUT8 | Homo sapiens |
additional information | A549/shFUT8, knockdown mutant of intrinsic FUT8, no enzyme activity, decrease of cellular growth in response to epidermal growth factor stimulation, decreased sensitivity of A549 cells to gefitinib | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
gefitinib | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | - |
Homo sapiens | - |
HEK-293 cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
44.1 U/l in HEK-293 cells transfected with epidermal growth factor receptor and FUT8, 1.8 U/l in HEK-293 cells transfected only with epidermal growth factor receptor | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine | pH 7.0, 37°C, 6 h, reaction stopped by heating at 100°C for 1 min | Homo sapiens | GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha1,6-fucosyltransferase | - |
Homo sapiens |
FUT8 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |