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Literature summary for 2.4.1.68 extracted from

  • Uozumi, N.; Teshima, T.; Yamamoto, T.; Nishikawa, A.; Gao, Y.E.; Miyoshi, E.; Gao, C.X.; Noda, K.; Islam, K.N.; et al.
    A fluorescent assay method for GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1-6fucosyltransferase activity, involving high performance liquid chromatography (1996), J. Biochem., 120, 385-392.
    View publication on PubMed
Search for more literature for 2.4.1.68

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0174
-
 N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) liver Sus scrofa
0.046
-
 GDP-L-fucose liver Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Sus scrofa
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from serum by affinity chromatography with a GDP-hexanolamine resin Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
brain
-
 Sus scrofa
-
hepatoma cell
-
 Homo sapiens
-
hepatoma cell DAB-induced hepatoma Rattus norvegicus
-
liver
-
 Homo sapiens
-
liver
-
 Sus scrofa
-
liver fetal and adult Rattus norvegicus
-
serum
-
 Homo sapiens
-
serum
-
 Rattus norvegicus
-
spleen
-
 Rattus norvegicus
-
Yoshida AH-130 cell
-
 Rattus norvegicus
-
Yoshida AH-66 cell
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens
additional information
-
 fluorescent assay method development, structural analysis of the reaction product, kinetics Rattus norvegicus
additional information
-
 fluorescent assay method development, structural analysis of the reaction product, kinetics Sus scrofa
0.00000017
-
 fetal liver Rattus norvegicus
0.00000026
-
 adult liver Rattus norvegicus
0.00000037
-
 hepatoma cell line AH-66 Rattus norvegicus
0.00000072
-
 DAB-induced hepatoma cell Rattus norvegicus
0.0000025
-
 hepatoma cell line AH-130 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine i.e. GnGn-peptide Homo sapiens GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine i.e. GnGn-peptide Rattus norvegicus GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine i.e. GnGn-peptide Sus scrofa GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGn-bi-Asn-PABA, synthetic substrate, fluorescent assay Homo sapiens GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGnF-bi-Asn-PABA ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGn-bi-Asn-PABA, synthetic substrate, fluorescent assay Rattus norvegicus GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGnF-bi-Asn-PABA ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGn-bi-Asn-PABA, synthetic substrate, fluorescent assay Sus scrofa GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGnF-bi-Asn-PABA ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) acceptor substrate is derived from bovine gamma-globulin Homo sapiens GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGnF-bi-Asn-PABA ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) acceptor substrate is derived from bovine gamma-globulin Rattus norvegicus GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGnF-bi-Asn-PABA ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) acceptor substrate is derived from bovine gamma-globulin Sus scrofa GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGnF-bi-Asn-PABA ?

Synonyms

Synonyms Comment Organism
alpha1-6FucT
-
 Homo sapiens
alpha1-6FucT
-
 Rattus norvegicus
alpha1-6FucT
-
 Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Sus scrofa
37
-
 assay at Homo sapiens
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 liver Rattus norvegicus
5.6
-
 liver microsomes Sus scrofa
6.5
-
 spleen Rattus norvegicus
7
-
-
 Sus scrofa
7
-
 brain and serum Rattus norvegicus