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Literature summary for 2.4.1.66 extracted from

  • Barber, A.J.; Jamieson, G.A.
    Platelet collagen adhesion characterization of collagen glucosyltransferase of plasma membranes of human blood platelets (1971), Biochim. Biophys. Acta, 252, 533-545.
    View publication on PubMed
Search for more literature for 2.4.1.66

Inhibitors

Inhibitors Comment Organism Structure
Acetylsalicylic acid aspirin Homo sapiens
ADP
-
 Homo sapiens
AMP
-
 Homo sapiens
cAMP 4.5 mM: 26% inhibition Homo sapiens
D-glucosamine
-
 Homo sapiens
additional information not inhibited by D-glucose, N-acetylglucosamine, N-acteylgalactosamine, Triton X-100, delta-hydroxylysine Homo sapiens
p-hydroxymercuribenzoate 1 mM: complete inhibition Homo sapiens
UDP product inhibition Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound Homo sapiens 16020
-
plasma membrane found primarily in Homo sapiens 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates, 20% as effective as Mn2+ Homo sapiens
Co2+ activates, 20% as effective as Mn2+ Homo sapiens
Mg2+ activates, about 20% as effective as Mn2+ Homo sapiens
Mn2+ requirement Homo sapiens
Mn2+ 15 mM Homo sapiens
additional information not activated by Cd2+ Homo sapiens
additional information not activated by Zn2+, Cu2+ Homo sapiens
additional information not activated by Na+, K+, Ba2+ Homo sapiens
additional information not activated by Fe3+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen Homo sapiens may play a major role in the mechanism of platelet:collagen adhesion UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
20fold Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood platelet
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: ovalbumin, transferrin, fetuin Homo sapiens ?
-
 ?
additional information not: bovine and ovine submaxillary glycoprotein, ovalbumin, ceruloplasmin, human albumin, fibrinogen, haptoglobin, deaminated collagen, very poor glucosyl donors: UDPgalactose, ADPglucose Homo sapiens ?
-
 ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen specific for collagen Homo sapiens UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen incomplete hexasaccharide chains of bovine Achilles tendon collagen, glucosyl residues removed by mild acid hydrolysis Homo sapiens UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen may play a major role in the mechanism of platelet:collagen adhesion Homo sapiens UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?
UDP-glucose + fetuin minus sialic acid, galactose and N-acetylglucosamine 7% as effective as glucose-free bovine Achilles tendon collagen Homo sapiens ?
-
 ?
UDP-glucose + transferrin minus Fe3+ and sialic acid 7% as effective as glucose-free bovine Achilles tendon collagen Homo sapiens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.7
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5.2 6.5 about half-maximal activity at pH 5.2 and 6.5 Homo sapiens