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Literature summary for 2.4.1.66 extracted from

  • Anttinen, H.; Myllylä, R.; Kivirikko, K.I.
    Further characterization of galactosylhydroxylysyl glucosyltransferase from chick embryos. Amino acid composition and acceptor specificity (1978), Biochem. J., 175, 737-742.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.66

General Stability

General Stability Organism
loses activity during purification mostly due to inactivation Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5
-
 D-galactosylsphingosine
-
 Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid composition Gallus gallus
70000
-
 about Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen Gallus gallus involved in collagen biosynthesis UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 white Leghorn chicken
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Gallus gallus

Purification (Commentary)

Purification (Comment) Organism
39800fold from embryo Gallus gallus
affinity chromatography Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
embryo 14-15 days old Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.57
-
-
 Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: casein, chondroitin sulfate, ovomucoid, alpha1-glycoprotein, alpha-lactalbumin, thyroglobulin Gallus gallus ?
-
 ?
additional information not: ovalbumin, transferrin, fetuin Gallus gallus ?
-
 ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen acceptor specificity Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen high specificity for galactosylhydroxylysine Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen heat-denatured citrate-soluble rat skin collagen Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen specific for collagen Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen calf skin gelatin Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen involved in collagen biosynthesis Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?
UDP-glucose + D-galactosylsphingosine in vitro Gallus gallus UDP + 1,2-D-glucosyl-D-galactosylsphingosine
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information 0.7 mol of glucosylgalactosylhydroxylysine residues formed per s per mol of enzyme Gallus gallus