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Literature summary for 2.4.1.66 extracted from

  • Myllylä, R.; Risteli, L.; Kivirikko, K.I.
    Collagen glucosyltransferase. Partial purification and characterization of the enzyme from whole chick embryos and chick-embryo cartilage (1976), Eur. J. Biochem., 61, 59-67.
    View publication on PubMed
Search for more literature for 2.4.1.66

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activation, optimal concentration: 1 mM Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
p-mercuribenzoate 0.0007 mM: 50% inhibition, substrates plus Mn2+ or Mn2+ alone partially protect Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum located within the cisternae of the endoplasmic reticulum Gallus gallus 5783
-
membrane associated with membrane structures Gallus gallus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation, 10 mM Gallus gallus
Ca2+ Mn2+ is the most effective metal cofactor, partial replacement with Co2+, Mg2+ and Ca2+ Gallus gallus
Co2+ 32-33% as effective as Mn2+ Gallus gallus
Co2+ Mn2+ is the most effective metal cofactor, partial replacement with Co2+, Mg2+ and Ca2+, optimal concentration: 2.5 mM Gallus gallus
Mg2+ Mn2+ is the most effective metal cofactor, partial replacement with Co2+, Mg2+ and Ca2+, optimal concentration: 30 mM Gallus gallus
Mn2+ requirement Gallus gallus
Mn2+ 10 mM Gallus gallus
Mn2+ partial replacement of Mn2+ with Co2+, Mg2+ and Ca2+, enzyme-Mn2+ is required for the binding of the substrate and co-substrate Gallus gallus
Mn2+ most effective metal cofactor Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000 54000 gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 white Leghorn chicken
-

Purification (Commentary)

Purification (Comment) Organism
over 2000fold from whole embryo, 160fold from embryo cartilage Gallus gallus
partial Gallus gallus
solubilized with Triton X-100 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
cartilage embryonic Gallus gallus
-
cartilage cartilage and cartilagenous bone Gallus gallus
-
embryo embryo cartilage Gallus gallus
-
embryo 14-15 days old Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0033
-
 enzyme from embryo cartilage Gallus gallus
0.031 0.041 enzyme from whole embryo Gallus gallus

Storage Stability

Storage Stability Organism
4°C, dilute enzyme solution, 1 week, more than 50% loss of activity Gallus gallus
frozen, concentrated purified enzyme solution, little loss of activity Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen heat-denatured citrate-soluble rat skin collagen Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen calf skin gelatin Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Gallus gallus