Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Salisediminibacterium selenitireducens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha,alpha-trehalose | - |
Salisediminibacterium selenitireducens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
phosphate | recombinant enzyme, at pH 6.5 and 30°C | Salisediminibacterium selenitireducens | |
0.86 | - |
alpha,alpha-trehalose | recombinant enzyme, at pH 6.5 and 30°C | Salisediminibacterium selenitireducens | |
2 | - |
beta-D-glucose 1-phosphate | recombinant enzyme, at pH 6.5 and 30°C | Salisediminibacterium selenitireducens | |
5.5 | - |
D-glucose | recombinant enzyme, at pH 6.5 and 30°C | Salisediminibacterium selenitireducens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | essential for enzymatic activity. Decreasing potassium ion concentrations significantly reduce thermal and pH stabilities of the enzyme | Salisediminibacterium selenitireducens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87889 | - |
2 * 87889, calculated from amino acid sequence | Salisediminibacterium selenitireducens |
88000 | - |
2 * 88000, SDS-PAGE | Salisediminibacterium selenitireducens |
170000 | - |
gel filtration | Salisediminibacterium selenitireducens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + phosphate | Salisediminibacterium selenitireducens | - |
D-glucose + beta-D-glucose 1-phosphate | - |
r | |
alpha,alpha-trehalose + phosphate | Salisediminibacterium selenitireducens MLS10 | - |
D-glucose + beta-D-glucose 1-phosphate | - |
r | |
D-glucose + beta-D-glucose 1-phosphate | Salisediminibacterium selenitireducens | - |
alpha,alpha-trehalose + phosphate | - |
r | |
D-glucose + beta-D-glucose 1-phosphate | Salisediminibacterium selenitireducens MLS10 | - |
alpha,alpha-trehalose + phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salisediminibacterium selenitireducens | D6XSD4 | - |
- |
Salisediminibacterium selenitireducens MLS10 | D6XSD4 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Salisediminibacterium selenitireducens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + phosphate | - |
Salisediminibacterium selenitireducens | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
alpha,alpha-trehalose + phosphate | - |
Salisediminibacterium selenitireducens MLS10 | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
D-glucose + beta-D-glucose 1-phosphate | - |
Salisediminibacterium selenitireducens | alpha,alpha-trehalose + phosphate | - |
r | |
D-glucose + beta-D-glucose 1-phosphate | - |
Salisediminibacterium selenitireducens MLS10 | alpha,alpha-trehalose + phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 88000, SDS-PAGE | Salisediminibacterium selenitireducens |
homodimer | 2 * 87889, calculated from amino acid sequence | Salisediminibacterium selenitireducens |
Synonyms | Comment | Organism |
---|---|---|
Bsel_1207 | recombinant protein | Salisediminibacterium selenitireducens |
trehalose phosphorylase | - |
Salisediminibacterium selenitireducens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
60 | - |
D-glucose | recombinant enzyme, at pH 6.5 and 30°C | Salisediminibacterium selenitireducens | |
68 | - |
beta-D-glucose 1-phosphate | recombinant enzyme, at pH 6.5 and 30°C | Salisediminibacterium selenitireducens | |
210 | - |
alpha,alpha-trehalose | recombinant enzyme, at pH 6.5 and 30°C | Salisediminibacterium selenitireducens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
95 | - |
alpha,alpha-trehalose | recombinant enzyme, at pH 6.5 and 30°C | Salisediminibacterium selenitireducens |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in osmotic control through the biosynthesis of trehalose | Salisediminibacterium selenitireducens |