Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermoanaerobacter brockii |
Protein Variants | Comment | Organism |
---|---|---|
A431T | the mutant shows strongly decreased catalytic efficiency for D-glucose and decreased catalytic efficiency for D-galactose compared to the wild type enzyme | Thermoanaerobacter brockii |
A440E | the mutant shows increased catalytic efficiency for D-glucose and wild type catalytic efficiency for D-galactose compared to the wild type enzyme | Thermoanaerobacter brockii |
A440K | the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme | Thermoanaerobacter brockii |
A440M | the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme | Thermoanaerobacter brockii |
A440V | the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme | Thermoanaerobacter brockii |
A440V/N657Y | the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme | Thermoanaerobacter brockii |
A440V/R448S | the mutant shows increased catalytic efficiency for D-glucose and strongly increased catalytic efficiency for D-galactose compared to the wild type enzyme | Thermoanaerobacter brockii |
N657D | the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme | Thermoanaerobacter brockii |
N657G | the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme | Thermoanaerobacter brockii |
N657I | the mutant shows approximate 2fold increase in affinity for D-galactose | Thermoanaerobacter brockii |
N657L | the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme | Thermoanaerobacter brockii |
N657R | the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme | Thermoanaerobacter brockii |
N657Y | the mutant shows approximate 2fold increase in affinity for D-galactose | Thermoanaerobacter brockii |
P588H | the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme | Thermoanaerobacter brockii |
R448D | the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme | Thermoanaerobacter brockii |
R448F | the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme | Thermoanaerobacter brockii |
R448N | the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme | Thermoanaerobacter brockii |
R448S | the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme | Thermoanaerobacter brockii |
R448S/N657Y | the mutant shows about wild type catalytic efficiencies for D-glucose and D-galactose | Thermoanaerobacter brockii |
R448V | the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme | Thermoanaerobacter brockii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
D-glucose | mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.6 | - |
D-glucose | mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.7 | - |
D-glucose | mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
1.2 | - |
D-glucose | mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
1.2 | - |
D-glucose | mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
1.3 | - |
D-glucose | mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
1.5 | - |
D-glucose | mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
1.5 | - |
D-glucose | mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
1.5 | - |
D-glucose | mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
1.8 | - |
D-glucose | mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
1.9 | - |
D-glucose | mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
1.9 | - |
D-glucose | mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
2.1 | - |
D-glucose | mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
2.1 | - |
D-glucose | mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
2.2 | - |
D-glucose | mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
2.3 | - |
D-glucose | mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
2.3 | - |
D-glucose | mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
2.5 | - |
D-glucose | mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
3 | - |
D-glucose | mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
3 | 3.5 | D-galactose | mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
3.2 | - |
D-glucose | wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
30.1 | - |
D-galactose | mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
35.1 | - |
D-galactose | mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
36.8 | - |
D-glucose | mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
38 | - |
D-galactose | mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
43.7 | - |
D-galactose | mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
43.8 | - |
D-galactose | mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
45.2 | - |
D-galactose | mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
45.7 | - |
D-galactose | mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
45.8 | - |
D-galactose | mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
47.5 | - |
D-galactose | mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
53.5 | - |
D-galactose | mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
53.6 | - |
D-galactose | mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
56.8 | - |
D-galactose | mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
58.1 | - |
D-galactose | mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
61.1 | - |
D-galactose | mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
61.9 | - |
D-galactose | mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
62.5 | - |
D-galactose | mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
68.2 | - |
D-galactose | mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
96 | - |
D-galactose | mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
98 | - |
D-galactose | wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
447.6 | - |
D-galactose | mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacter brockii | Q8L164 | previously known as Thermoanaerobium brockii | - |
Purification (Comment) | Organism |
---|---|
- |
Thermoanaerobacter brockii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose 1-phosphate + D-galactose | low activity. The Km value for D-galactose is about 30fold higher than that for D-glucose | Thermoanaerobacter brockii | ? + phosphate | - |
r | |
D-glucose + beta-D-glucose 1-phosphate | best substrate combination | Thermoanaerobacter brockii | alpha,alpha-trehalose + phosphate | - |
r | |
additional information | the wild type enzyme shows low activity with D-fructose and intermediate activity with D-mannose | Thermoanaerobacter brockii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
trehalose phosphorylase | - |
Thermoanaerobacter brockii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the half-life of the wild type enzyme remains constant for about 13 h at 60°C | Thermoanaerobacter brockii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7 | - |
D-glucose | mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
14 | - |
D-galactose | mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
16.1 | - |
D-glucose | mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
17 | - |
D-galactose | mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
18.6 | - |
D-glucose | mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
21.1 | - |
D-galactose | mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
21.7 | - |
D-glucose | mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
22.6 | - |
D-galactose | mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
23 | - |
D-galactose | mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
23.3 | - |
D-galactose | mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
23.6 | - |
D-galactose | mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
24 | - |
D-galactose | mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
25 | - |
D-glucose | mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
26.2 | - |
D-galactose | mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
26.3 | - |
D-glucose | mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
27.4 | - |
D-glucose | mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
27.7 | - |
D-galactose | mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
30.6 | - |
D-glucose | mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
31.4 | - |
D-glucose | mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
31.9 | - |
D-galactose | mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
32.1 | - |
D-glucose | mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
32.4 | - |
D-galactose | mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
32.7 | - |
D-glucose | mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
32.9 | - |
D-galactose | mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
32.9 | - |
D-galactose | wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
33.3 | - |
D-galactose | mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
33.9 | - |
D-glucose | wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
35.5 | - |
D-galactose | mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
36.1 | - |
D-glucose | mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
38.3 | - |
D-galactose | mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
38.9 | - |
D-galactose | mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
41.2 | - |
D-glucose | mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
42 | - |
D-glucose | mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
42.2 | - |
D-galactose | mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
42.7 | - |
D-glucose | mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
43.3 | - |
D-glucose | mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
43.5 | - |
D-glucose | mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
46.9 | - |
D-glucose | mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
51.2 | - |
D-glucose | mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
53.9 | - |
D-glucose | mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
54.3 | - |
D-galactose | mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
66.5 | - |
D-galactose | mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.149 | - |
D-galactose | mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.336 | - |
D-galactose | wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.337 | - |
D-galactose | mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.368 | - |
D-galactose | mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.388 | - |
D-galactose | mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.413 | - |
D-galactose | mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.444 | - |
D-galactose | mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.488 | - |
D-galactose | mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.5 | - |
D-galactose | mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.509 | - |
D-galactose | mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.545 | - |
D-galactose | mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.566 | - |
D-galactose | mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.574 | - |
D-galactose | mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.6 | - |
D-galactose | mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.604 | - |
D-galactose | mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.613 | - |
D-galactose | mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.672 | - |
D-galactose | mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.72 | - |
D-galactose | mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.789 | - |
D-galactose | mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.872 | - |
D-glucose | mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
0.952 | - |
D-galactose | mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
1.29 | - |
D-galactose | mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
11 | - |
D-glucose | wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
12 | - |
D-glucose | mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
13 | - |
D-glucose | mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
14 | - |
D-glucose | mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
16 | - |
D-glucose | mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
17 | - |
D-glucose | mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
18 | - |
D-glucose | mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
18 | - |
D-glucose | mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
18 | - |
D-glucose | mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
18 | - |
D-glucose | mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
19 | - |
D-glucose | mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
20 | - |
D-glucose | mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
20 | - |
D-glucose | mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
22 | - |
D-glucose | mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
22 | - |
D-glucose | mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
24 | - |
D-glucose | mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
27 | - |
D-glucose | mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
28 | - |
D-glucose | mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
29 | - |
D-glucose | mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii | |
36 | - |
D-glucose | mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C | Thermoanaerobacter brockii |