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Literature summary for 2.4.1.64 extracted from
- Purification and characterization of thermostable trehalose phosphorylase from Thermoanaerobium brockii (1999), J. Appl. Glycosci., 46, 399-405.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Thermoanaerobacter brockii |  |
| Hg2+ | - |
Thermoanaerobacter brockii | |
| Mg2+ | - |
Thermoanaerobacter brockii | |
| Mn2+ | - |
Thermoanaerobacter brockii | |
| Pb2+ | - |
Thermoanaerobacter brockii | |
| Zn2+ | - |
Thermoanaerobacter brockii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.57 | - |
phosphate | - |
Thermoanaerobacter brockii | |
| 0.75 | - |
beta-D-glucose 1-phosphate | - |
Thermoanaerobacter brockii | |
| 0.97 | - |
trehalose | - |
Thermoanaerobacter brockii | |
| 2.4 | - |
D-glucose | - |
Thermoanaerobacter brockii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88000 | - |
x * 88000, SDS-PAGE | Thermoanaerobacter brockii |
| 190000 | - |
gel filtration | Thermoanaerobacter brockii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoanaerobacter brockii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoanaerobacter brockii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha,alpha-trehalose + phosphate | - |
Thermoanaerobacter brockii | D-glucose + beta-D-glucose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 88000, SDS-PAGE | Thermoanaerobacter brockii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
both directions | Thermoanaerobacter brockii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable up to | Thermoanaerobacter brockii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | synthesis of trehalose | Thermoanaerobacter brockii |
| 7 | 7.5 | phosphorolysis of trehalose | Thermoanaerobacter brockii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | stable | Thermoanaerobacter brockii |
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