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Literature summary for 2.4.1.62 extracted from
- Mouse beta 1,3-galactosyltransferase (GA1/GM1/GD1b synthase): protein characterization, tissue expression, and developmental regulation in neural retina (1999), J. Neurosci. Res., 58, 318-327.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning and DNA sequence determination and analysis, expression in CHO-K1 and NIH-3T3 cells as fusion protein tagged with a nonapeptide from influenza virus hemagglutinin, amino acid sequence determination | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.023 | - |
N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine | recombinant fusion protein | Mus musculus |  |
| 2.47 | - |
UDP-galactose | recombinant fusion protein | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi apparatus | recombinant enzyme, expressed in CHO-K1 cells | Mus musculus | 5794 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid sequence alignment | Mus musculus |
| 43000 | - |
x * 43000, recombinant enzyme from CHO-K1 cells, SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-galactose + ganglioside GA2 | Mus musculus | i.e. Cer-Glc-Gal-GalNAc | UDP + ganglioside GA1 | i.e. Cer-Glc-Gal-GalNAc-Gal | ? | |
| UDP-galactose + GD2 | Mus musculus | i.e. Cer-Glc-Gal-(GalNAc)(NeuNAc2) | UDP + Gd1b | i.e. Cer-Glc-Gal-(GalNAc-Gal)(NeuNAc2) | ? | |
| UDPgalactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine | Mus musculus | involved in biosynthesis of gangliosides | UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine | i.e. GM1 | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q9Z0F0 | single gene | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | N-glycosylated at the unique glycosylation site | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Mus musculus | - |
| kidney | - |
Mus musculus | - |
| lung | low activity content | Mus musculus | - |
| neural retina | expression level depends on the developmental stage | Mus musculus | - |
| spleen | - |
Mus musculus | - |
| testis | highest content of activity | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00000005 | - |
recombinant enzyme, crude cell homogenate of transfected NIH 3T3 cells | Mus musculus |
| 0.00000007 | - |
recombinant enzyme, crude cell homogenate of transfected CHO-K1 cells | Mus musculus |
| 0.00000009 | - |
testis, crude homogenate, native wild-type | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-galactose + ganglioside GA2 | i.e. Cer-Glc-Gal-GalNAc | Mus musculus | UDP + ganglioside GA1 | i.e. Cer-Glc-Gal-GalNAc-Gal | ? | |
| UDP-galactose + GD2 | i.e. Cer-Glc-Gal-(GalNAc)(NeuNAc2) | Mus musculus | UDP + Gd1b | i.e. Cer-Glc-Gal-(GalNAc-Gal)(NeuNAc2) | ? | |
| UDP-galactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine | - |
Mus musculus | UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine | i.e. GM1 | ? | |
| UDPgalactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine | involved in biosynthesis of gangliosides | Mus musculus | UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine | i.e. GM1 | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, recombinant enzyme from CHO-K1 cells, SDS-PAGE | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GA1/GM1/GD1b synthase | - |
Mus musculus |
| Gal-T2 | - |
Mus musculus |
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