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Literature summary for 2.4.1.50 extracted from

  • Risteli, L.
    Further characterization of collagen galactosyltransferase from chick embryos (1978), Biochem. J., 169, 189-196.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 20 mM, activates Gallus gallus
Nonidet P-40 activates Gallus gallus
Triton X-100 0.1%, 2fold stimulation Gallus gallus
Urea 2 mM, activates Gallus gallus

General Stability

General Stability Organism
highly labile enzyme, DTT stabilizes during purification, 0.25 M or 0.1 M galactose or glycerol 25% v/v do not stabilize Gallus gallus
unstable during purification Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
Gallus gallus
AMP
-
Gallus gallus
ATP
-
Gallus gallus
CDP
-
Gallus gallus
Co2+ 6 mM: 50% inhibition Gallus gallus
concanavalin A 0.3 M methyl-alpha-D-glucopyranoside protects, but not galactose Gallus gallus
Galactosylhydroxylysine
-
Gallus gallus
GDP
-
Gallus gallus
gelatinized calf skin collagen above 60 mg/ml Gallus gallus
human renal basement membrane collagen glucose-free Gallus gallus
hydroxylysine weak Gallus gallus
Mn2+ above 2.5 mM Gallus gallus
additional information not inhibited by lysine, free monosaccharides, 1 mM lactose, 1 mM sucrose, 2 mM uridine Gallus gallus
UDP
-
Gallus gallus
UDPgalacturonate
-
Gallus gallus
UDPglucose
-
Gallus gallus
UDPglucuronate
-
Gallus gallus
UMP
-
Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km: 16 mg/ml, gelatinized calf skin collagen Gallus gallus
0.02 0.023 UDPgalactose
-
Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum cisterna presumed location within Gallus gallus
-
-
membrane associated with membrane structures Gallus gallus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ requirement Gallus gallus
Mn2+ optimal concentration: 2-2.5 mM Gallus gallus
additional information not activated by Co2+ Gallus gallus
additional information not activated by Mg2+ Gallus gallus
additional information not activated by Fe2+ Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Gallus gallus collagen biosynthesis ?
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Ethylene glycol 50% v/v, stable to Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
white Leghorn chicken
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein presence of carbohydrate units in enzyme molecule Gallus gallus

Purification (Commentary)

Purification (Comment) Organism
250-300fold Gallus gallus
optimal extraction requires high concentrations of detergent and salt Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
embryo 14-15 days old Gallus gallus
-

Storage Stability

Storage Stability Organism
-20°C, 50% v/v ethylene glycol, several weeks, stable Gallus gallus
4°C, unstable to storage, urea, galactose, Triton X-100, glycerol or ethylene glycol do not stabilize Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no acceptor: free hydroxylysine Gallus gallus ?
-
?
additional information collagen biosynthesis Gallus gallus ?
-
?
UDPgalactose + procollagen 5-hydroxy-L-lysine gelatinized insoluble calf skin collagen Gallus gallus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Gallus gallus