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Literature summary for 2.4.1.50 extracted from

  • Risteli, L.; Myllylä, R.; Kivirikko, K.I.
    Partial purification and characterization of collagen galactosyltransferase from chick embryos (1976), Biochem. J., 155, 145-153.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.50

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 0.5-5 mM: 20-30% activation Gallus gallus
Triton X-100 0.1%, 2fold stimulation Gallus gallus

General Stability

General Stability Organism
unstable during purification Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
p-mercuribenzoate strong, 0.007 mM: 50% inhibition Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km: 2-4 mg/ml, denatured citrate-soluble rat skin collagen Gallus gallus
additional information
-
 additional information Km: 35-70 mg/ml, gelatinized insoluble calf skin collagen Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated with membrane structures Gallus gallus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 3 species of enzyme activity: 450000, 200000 and 50000 Da, gel filtration Gallus gallus
200000
-
 3 species of enzyme activity: 450000, 200000 and 50000 Da, gel filtration Gallus gallus
450000
-
 3 species of enzyme activity: 450000, 200000 and 50000 Da, possibly an aggregate of enzyme or enzyme with other proteins, gel filtration Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Gallus gallus intracellular post-translational modification in collagen biosynthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 white Leghorn chicken
-

Purification (Commentary)

Purification (Comment) Organism
50-150fold Gallus gallus
partial Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
cartilage
-
 Gallus gallus
-
embryo 14-15 days old Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00093
-
-
 Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no acceptor: native collagen Gallus gallus ?
-
 ?
additional information free thiol groups in the active centre Gallus gallus ?
-
 ?
additional information intracellular post-translational modification in collagen biosynthesis Gallus gallus ?
-
 ?
UDPgalactose + procollagen 5-hydroxy-L-lysine triple helix conformation of native collagen at 30°C prevents glycosylation Gallus gallus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
 ?
UDPgalactose + procollagen 5-hydroxy-L-lysine peptides derived from citrate soluble collagen by collagenase digestion, longer peptides are better substrates Gallus gallus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
 ?
UDPgalactose + procollagen 5-hydroxy-L-lysine heat-denatured citrate-soluble rat skin collagen is a more effective substrate than gelatinized insoluble calf skin collagen Gallus gallus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
 ?
UDPgalactose + procollagen 5-hydroxy-L-lysine gelatinized insoluble calf skin collagen Gallus gallus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
 ?
UDPgalactose + submaxillary glycoprotein with sialic acid removed not porcine Gallus gallus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Gallus gallus