Activating Compound | Comment | Organism | Structure |
---|---|---|---|
exopolysaccharides | stimulate, whereby concentrations of 0.5% cause the strongest effects of up to 1.3fold | Limosilactobacillus reuteri | |
exopolysaccharides | stimulate, whereby concentrations of 0.5% cause the strongest effects of up to 1.5-2fold | Latilactobacillus curvatus | |
fructan | activates the enzyme | Ligilactobacillus animalis | |
Glucan | - |
Latilactobacillus curvatus | |
Glucan | - |
Limosilactobacillus reuteri | |
Glucan | activates the enzyme | Ligilactobacillus animalis | |
additional information | the enzyme is strongly activated through its own exopolysaccharide by 1.5-2fold. The fructan of Lactobacillus sanfranciscensis has either no effect or an inhibitory one | Ligilactobacillus animalis |
Application | Comment | Organism |
---|---|---|
food industry | the exopolysaccharides of Lactobacillus animalis TMW 1.971 improve the quality of gluten-free breads, they can be produced in situ to levels enabling baking applications | Ligilactobacillus animalis |
food industry | the exopolysaccharides of Lactobacillus curvatus TMW 1.624 improve the quality of gluten-free breads, they can be produced in situ to levels enabling baking applications | Latilactobacillus curvatus |
food industry | the exopolysaccharides of Lactobacillus reuteri TMW 1.106 improve the quality of gluten-free breads, they can be produced in situ to levels enabling baking applications | Limosilactobacillus reuteri |
Cloned (Comment) | Organism |
---|---|
gene gtf, recombinant expression in Escherichia coli strain Rosetta | Latilactobacillus curvatus |
gene gtf, recombinant expression in Escherichia coli strain Rosetta | Limosilactobacillus reuteri |
gene gtf, recombinant expression in Escherichia coli strain Rosetta | Ligilactobacillus animalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Latilactobacillus curvatus | |
Cu2+ | - |
Ligilactobacillus animalis | |
Cu2+ | - |
Limosilactobacillus reuteri | |
EDTA | - |
Latilactobacillus curvatus | |
EDTA | - |
Ligilactobacillus animalis | |
fructan | the fructan of Lactobacillus sanfranciscensis has either no effect or an inhibitory effect | Ligilactobacillus animalis | |
Mn2+ | inhibits hydrolysis activity almost entirely, but activates transferase activity at 1 mM | Limosilactobacillus reuteri | |
Zn2+ | inhibits hydrolysis activity but has no effect on transferase activity which constitutes to more than 90% of the overall activity, Zn2+ has therefore no influence on overall activity | Latilactobacillus curvatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Ligilactobacillus animalis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates at 1 mM, best activating metal ion | Latilactobacillus curvatus | |
Ca2+ | activates at 1 mM, best activating metal ion | Limosilactobacillus reuteri | |
Ca2+ | activates at 1 mM, best activating metal ion | Ligilactobacillus animalis | |
K+ | activates at 1 mM | Latilactobacillus curvatus | |
K+ | activates at 1 mM | Ligilactobacillus animalis | |
Mg2+ | activates at 1 mM | Ligilactobacillus animalis | |
Mn2+ | activates at 1 mM | Latilactobacillus curvatus | |
Mn2+ | inhibits hydrolysis activity almost entirely, but activates transferase activity at 1 mM | Limosilactobacillus reuteri | |
additional information | Mn2+ has no influence on hydrolysis and transferase activity. Al3+ and Na+ also do not significantly influence overall activity | Ligilactobacillus animalis | |
additional information | no effect by 1 mM EDTA | Limosilactobacillus reuteri | |
Na+ | activates at 1 mM | Latilactobacillus curvatus | |
Zn2+ | activates at 1 mM | Limosilactobacillus reuteri | |
Zn2+ | activates at 1 mM | Ligilactobacillus animalis | |
Zn2+ | inhibits hydrolysis activity but has no effect on transferase activity which constitutes to more than 90% of the overall activity, Zn2+ has therefore no influence on overall activity. Al3+ and Mg2+ also have no significant influence on activity | Latilactobacillus curvatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
183630 | - |
x * 183630, sequence calculation | Latilactobacillus curvatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + [(1->6)-alpha-D-glucosyl]n | Latilactobacillus curvatus | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | Limosilactobacillus reuteri | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | Ligilactobacillus animalis | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | Latilactobacillus curvatus TMW 1.624 | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | Limosilactobacillus reuteri TMW 1.106 | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | Ligilactobacillus animalis TMW 1.971 | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Latilactobacillus curvatus | - |
gene gtf | - |
Latilactobacillus curvatus TMW 1.624 | - |
gene gtf | - |
Ligilactobacillus animalis | - |
gene gtf | - |
Ligilactobacillus animalis TMW 1.971 | - |
gene gtf | - |
Limosilactobacillus reuteri | - |
gene gtf | - |
Limosilactobacillus reuteri TMW 1.106 | - |
gene gtf | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | growth and exopolysaccharide production correlated strongly for strain TMW 1.106, as soon as the strains reaches the stationary phase, exopolysaccharide production decreases and stops shortly afterward | Limosilactobacillus reuteri | - |
additional information | growth and exopolysaccharide production correlated strongly for strain TMW 1.624, as soon as the strains reaches the stationary phase, exopolysaccharide production decreases and stops shortly afterward | Latilactobacillus curvatus | - |
additional information | growth and exopolysaccharide production correlated strongly for strain TMW 1.971, as soon as the strains reaches the stationary phase, exopolysaccharide production decreases and stops shortly afterward | Ligilactobacillus animalis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Latilactobacillus curvatus | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Limosilactobacillus reuteri | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Ligilactobacillus animalis | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Latilactobacillus curvatus | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | structural properties of exopolysaccharides produced, overview | ? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Limosilactobacillus reuteri | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | structural properties of exopolysaccharides produced, overview | ? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Ligilactobacillus animalis | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | structural properties of exopolysaccharides produced, overview | ? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Latilactobacillus curvatus TMW 1.624 | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Latilactobacillus curvatus TMW 1.624 | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | structural properties of exopolysaccharides produced, overview | ? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Limosilactobacillus reuteri TMW 1.106 | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Limosilactobacillus reuteri TMW 1.106 | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | structural properties of exopolysaccharides produced, overview | ? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Ligilactobacillus animalis TMW 1.971 | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Ligilactobacillus animalis TMW 1.971 | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | structural properties of exopolysaccharides produced, overview | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 183630, sequence calculation | Latilactobacillus curvatus |
More | the enzyme contains conserved and less-conserved YG-repeats | Latilactobacillus curvatus |
Synonyms | Comment | Organism |
---|---|---|
Gtf | - |
Latilactobacillus curvatus |
Gtf | - |
Limosilactobacillus reuteri |
Gtf | - |
Ligilactobacillus animalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
31 | - |
- |
Latilactobacillus curvatus |
42 | - |
transferase activity, two temperature maxima at 42°C and 53°C for overall activity | Ligilactobacillus animalis |
45 | - |
- |
Limosilactobacillus reuteri |
53 | - |
hydrolysis activity, two temperature maxima at 42°C and 53°C for overall activity | Ligilactobacillus animalis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 38 | activity range, inactive at 40°C, profile overview | Latilactobacillus curvatus |
22 | 59 | more than 50% of maximal activity over the broad temperature profile | Ligilactobacillus animalis |
25 | 53 | activity range, profile overview | Limosilactobacillus reuteri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.4 | - |
- |
Latilactobacillus curvatus |
4.4 | - |
- |
Limosilactobacillus reuteri |
5.8 | - |
- |
Ligilactobacillus animalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.8 | 7.2 | broad range with activities higher than 60% of maximal activity | Ligilactobacillus animalis |
General Information | Comment | Organism |
---|---|---|
evolution | sequence comparisons of the four conserved regions I, II, III and IV in the catalytic domains of different Lactobacillus dextransucrases | Latilactobacillus curvatus |
evolution | sequence comparisons of the four conserved regions I, II, III and IV in the catalytic domains of different Lactobacillus dextransucrases | Limosilactobacillus reuteri |
evolution | sequence comparisons of the four conserved regions I, II, III and IV in the catalytic domains of different Lactobacillus dextransucrases | Ligilactobacillus animalis |
metabolism | growth and exopolysaccharide production correlated strongly for strain TMW 1.106 | Limosilactobacillus reuteri |
metabolism | growth and exopolysaccharide production correlated strongly for strain TMW 1.624 | Latilactobacillus curvatus |
metabolism | growth and exopolysaccharide production correlated strongly for strain TMW 1.971 | Ligilactobacillus animalis |
additional information | lactate is formed during production of exopolysaccharides | Latilactobacillus curvatus |
additional information | lactate is formed during production of exopolysaccharides | Limosilactobacillus reuteri |
additional information | Lactobacillus animalis is a strong acidifier. The pH decreases fast to pH 3.75 and after 30 h fermentation, 167.14 mM lactate is formed during production of exopolysaccharides | Ligilactobacillus animalis |