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Literature summary for 2.4.1.5 extracted from

  • Purama, R.K.; Agrawal, M.; Goyal, A.
    Stabilization of dextransucrase from Leuconostoc mesenteroides NRRL B-640 (2010), Indian J. Microbiol., 50 Suppl 1, 57-61.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
enzyme deactivation follows first order rate kinetics Leuconostoc mesenteroides
glutaraldehyde does not support the stability, rather it inhibits the enzyme activity, also glycerol, PEG 8000, and dextran (500 kDa) do not stabilize the enzyme Leuconostoc mesenteroides
Tween 80 stabilizes the purified enzyme, maximally at 30°C Leuconostoc mesenteroides

Inhibitors

Inhibitors Comment Organism Structure
EDTA 50% inhibition at 1 mM Leuconostoc mesenteroides
Glutaraldehyde does not support enzyme stability, but inhibits the enzyme activity Leuconostoc mesenteroides
Urea denatures the enzyme and causes 45%, 90% and 98% loss of activity within 30 min when treated at 1 M, 3 M, and 5 M concentration, respectively Leuconostoc mesenteroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information enzyme deactivation follows fi rst order rate kinetics Leuconostoc mesenteroides

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 activates slightly by 8% at 1 mM Leuconostoc mesenteroides
CoCl2 activates 22% at 4 mM Leuconostoc mesenteroides
MgCl2 activates slightly by 6% at 1 mM Leuconostoc mesenteroides

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
-
-
-
Leuconostoc mesenteroides NRRL B-640
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by PEG 1500 fractionation Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23
-
purified enzyme, pH 5.4, 30°C Leuconostoc mesenteroides

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, loss of 25% activity within 14 days at 30C Leuconostoc mesenteroides
4°C, purified enzyme, loss of 92% activity within 4 days at 30°C Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Leuconostoc mesenteroides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 30 purified enzyme, stable at, rapid loss of activity above. Loss of 65% activity within 20 h at 30°C without added stabilizer, in presence of Tween 80 the enzyme loses only 8% activity. Loss of 92% activity at 30°C within 4 days Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
assay at Leuconostoc mesenteroides