Literature summary for 2.4.1.5 extracted from
Ryu, H.; Jin, X.; Lee, J.; Woo, H.; Kim, Y.; Kim, G.; Seo, E.; Kang, H.; Kim, J.; Cho, D.; Kimura, A.; Kim, D.
Optimal expression and characterization of a fusion enzyme having dextransucrase and dextranase activities (2010), Enzyme Microb. Technol., 47, 212-215.
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
gene dsxr, the recombinant chimeric dsxr encodes dextranase-dextransucrase, DXSR, is sequentially composed of the dextransucrase-encoding gene dsrBCB4 and the gene dex2, encoding the dextranase from Arthrobacter oxidans, UniProt ID A9UKG4, expression in Escherichia coli strain BL21(DE3)pLysS |
Leuconostoc mesenteroides |
Protein Variants
Protein Variants |
Comment |
Organism |
additional information |
construction and optimal expression of a fusion enzyme DSXR having dextransucrase and dextranase activities, for optimization of protein expression, response surface methodology is used |
Leuconostoc mesenteroides |
Organism
Organism |
UniProt |
Comment |
Textmining |
Leuconostoc mesenteroides |
D2CFL0 |
gene dsrBCB4 |
- |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
additional information |
- |
120.1 U/ml, recombinant dextransucrase activity of recombinant DSXR chimeric enzyme, pH 5.8, 28°C |
Leuconostoc mesenteroides |
1.2 |
- |
purified recombinant chimeric DSXR, dextransucrase activity, pH 5.8, 28°C |
Leuconostoc mesenteroides |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
28 |
- |
assay at |
Leuconostoc mesenteroides |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
5.2 |
- |
- |
Leuconostoc mesenteroides |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
5 |
6.4 |
highly reduced activity below pH 5.0 and above pH 6.4 |
Leuconostoc mesenteroides |