Cloned (Comment) | Organism |
---|---|
gene dsrX, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain DH5alpha, expressed as a N-terminal His6-tagged protein Escherichia coli strain BL21(DE3), optimization of the recombinant expression system | Leuconostoc mesenteroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | strongly inhibits the native and the recombinant enzyme | Leuconostoc mesenteroides | |
Cu2+ | strongly inhibits the native and the recombinant enzyme | Leuconostoc mesenteroides | |
EDTA | inhibits the native and the recombinant enzyme | Leuconostoc mesenteroides | |
Fe2+ | slightly inhibits the native, but not the recombinant enzyme | Leuconostoc mesenteroides | |
Mg2+ | slightly inhibits the native, but not the recombinant enzyme | Leuconostoc mesenteroides | |
Na+ | slightly inhibits the native, but not the recombinant enzyme | Leuconostoc mesenteroides | |
Ni2+ | slightly inhibits the native and the recombinant enzyme | Leuconostoc mesenteroides | |
SDS | inhibits the native and the recombinant enzyme | Leuconostoc mesenteroides | |
Zn2+ | slightly inhibits the native and the recombinant enzyme | Leuconostoc mesenteroides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | slightly activates the native and the recombinant enzyme | Leuconostoc mesenteroides | |
Mn2+ | activates the native and the recombinant enzyme | Leuconostoc mesenteroides | |
additional information | the enzyme is not or poorly affected by Ca2+, K+, and pepstatin | Leuconostoc mesenteroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
167570 | - |
x * 167570, recombinant His6-tagged DsrX, sequence calculation | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
gene dsrX | - |
Leuconostoc mesenteroides CGMCC 1.544 | - |
gene dsrX | - |
Purification (Comment) | Organism |
---|---|
native enzyme partially by ammonium sulfate fractionation and dialysis, recombinant N-terminally His6-tagged DsrX from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Leuconostoc mesenteroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
97.37 | - |
recombinant enzyme in crude enzyme extract | Leuconostoc mesenteroides |
Subunits | Comment | Organism |
---|---|---|
? | x * 167570, recombinant His6-tagged DsrX, sequence calculation | Leuconostoc mesenteroides |
Synonyms | Comment | Organism |
---|---|---|
DsrX | - |
Leuconostoc mesenteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant enzyme | Leuconostoc mesenteroides |
40 | - |
native enzyme | Leuconostoc mesenteroides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 60 | the native DsrX shows higher stability than the recombinant DsrX at the temperature range from 4°C to 60°C for 2 h, native enzyme shows 84% remaining activity at 40°C after 2 h, while recombinant enzyme shows only 3.1% | Leuconostoc mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
native and recombinant enzyme | Leuconostoc mesenteroides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | - |
Leuconostoc mesenteroides |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 5 | purified recombinant enzyme, quite stable | Leuconostoc mesenteroides |