Cloned (Comment) | Organism |
---|---|
expression of the His-tagged enzyme from strain B-512F in Escherichia coli | Leuconostoc mesenteroides |
General Stability | Organism |
---|---|
the enzyme can be stabilized by high-molecular-weight dextran, polyethyleneglycol or nonionic detergents such as Tween 80 | Leuconostoc mesenteroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4,6-trinitrobenzene-sulphonic acid | inactivation | Leuconostoc mesenteroides | |
Cu2+ | - |
Leuconostoc mesenteroides | |
diethyldicarbonate | - |
Leuconostoc mesenteroides | |
EDTA | inactivation, reversible by Ca2+ | Leuconostoc mesenteroides | |
Fe3+ | - |
Leuconostoc mesenteroides | |
Mn2+ | - |
Leuconostoc mesenteroides | |
o-phthalaldehyde | inactivation | Leuconostoc mesenteroides | |
pyridoxal-5'-phosphate | inactivation | Leuconostoc mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
sucrose | 30°C | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
- |
- |
Leuconostoc mesenteroides B-512F | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography. Various methods such as precipitation by ammonium sulphate, ethanol, or polyethylene glycol, phase partitioning, ultrafiltration and chromatography are used to purify the enzyme, detailed overview. Purification of dextransucrase is rendered difficult by the presence of viscous dextran in the medium | Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | glucose transfer from sucrose to dextrans leading to polymerization with release of D-fructose, the enzyme contains an active site His residue as well as an essential lysine residue | Leuconostoc mesenteroides | ? | - |
? | |
additional information | glucose transfer from sucrose to dextrans leading to polymerization with release of D-fructose, the enzyme contains an active site His residue as well as an essential lysine residue | Leuconostoc mesenteroides B-512F | ? | - |
? | |
sucrose + (1,6-alpha-D-glucosyl)n | - |
Leuconostoc mesenteroides | D-fructose + (1,6-alpha-D-glucosyl)n+1 | - |
? | |
sucrose + (1,6-alpha-D-glucosyl)n | - |
Leuconostoc mesenteroides B-512F | D-fructose + (1,6-alpha-D-glucosyl)n+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the glycoside hydrolase family 70 | Leuconostoc mesenteroides |
sucrose:1, 6-alpha-D-glucan 6-alpha-glucosyltransferase | - |
Leuconostoc mesenteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Leuconostoc mesenteroides |