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Literature summary for 2.4.1.5 extracted from

  • Kim, D.; Robyt, J.F.; Lee, S.Y.; Lee, J.H.; Kim, Y.M.
    Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of Leuconostoc mesenteroides B-512FMCM dextransucrase (2003), Carbohydr. Res., 338, 1183-1189.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
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B-512FMCM
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Leuconostoc mesenteroides B-512FMCM
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B-512FMCM
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (1,6-alpha-D-glucosyl)n with increasing concentrations of sucrose, from 0.1 to 4.0 M, the amount of high-molecular weight dextran decreases with a concomitant increase in low-molecular weight dextran. At 0.1 M sucrose, pH 5.5, and 28°C, 99.8% of the dextran had a MW of more than 1000000 Da and at 4.0 M sucrose, 69.9% have a MW below 100000 Da and 30.1% have a MW of more than 1000000 Da, giving a bimodal distribution. The degree of branching increased from 5% for 0.1 M sucrose to 16.6% for 4.0 M sucrose. The temperature has very little effect on the size of the dextran, which is above 1000000 Da, but it has a significant effect on the degree of branching, which is 4.8% at 4 °C and increases to 14.7% at 45°C. Both the molecular weight and the degree of branching are not significantly affected by different pH values between 4.5 and 6.0 Leuconostoc mesenteroides D-fructose + (1,6-alpha-D-glucosyl)n+1
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sucrose + (1,6-alpha-D-glucosyl)n with increasing concentrations of sucrose, from 0.1 to 4.0 M, the amount of high-molecular weight dextran decreases with a concomitant increase in low-molecular weight dextran. At 0.1 M sucrose, pH 5.5, and 28°C, 99.8% of the dextran had a MW of more than 1000000 Da and at 4.0 M sucrose, 69.9% have a MW below 100000 Da and 30.1% have a MW of more than 1000000 Da, giving a bimodal distribution. The degree of branching increased from 5% for 0.1 M sucrose to 16.6% for 4.0 M sucrose. The temperature has very little effect on the size of the dextran, which is above 1000000 Da, but it has a significant effect on the degree of branching, which is 4.8% at 4 °C and increases to 14.7% at 45°C. Both the molecular weight and the degree of branching are not significantly affected by different pH values between 4.5 and 6.0 Leuconostoc mesenteroides B-512FMCM D-fructose + (1,6-alpha-D-glucosyl)n+1
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