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Literature summary for 2.4.1.5 extracted from

  • Kobayashi, M.; Matsuda, K.
    Purification and characterization of two activities of the intracellular dextransucrase from Leuconostoc mesenteroides NRRL B-1299 (1975), Biochim. Biophys. Acta, 397, 69-79.
    View publication on PubMed
Search for more literature for 2.4.1.5

Activating Compound

Activating Compound Comment Organism Structure
Dextrans addition of the B-1299 water-soluble dextan stimulates activity of enzyme I, no effect on activity of enzyme II Leuconostoc mesenteroides

Inhibitors

Inhibitors Comment Organism Structure
EDTA no effect Leuconostoc mesenteroides
Fe3+ enzyme I Leuconostoc mesenteroides
Guanidine-HCl 0.05 M, 60% inhibition Leuconostoc mesenteroides
Mn2+ enzyme I and II Leuconostoc mesenteroides
phenylmercuric acetate 1.0 mM, strong inhibition Leuconostoc mesenteroides
SDS 1.0 mM, strong inhibition Leuconostoc mesenteroides
Urea enzyme I loses 50% of its activity at 2.4 M urea, enzyme II is inhibited to 50% by 1.7 M urea Leuconostoc mesenteroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10.7
-
 sucrose enzyme I Leuconostoc mesenteroides

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM slight activation of enzyme I and II Leuconostoc mesenteroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
 1 * 69000, enzyme I, SDS-PAGE Leuconostoc mesenteroides
79000
-
 1 * 79000, enzyme II, SDS-PAGE Leuconostoc mesenteroides

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
-
 NRRL B-1299
-
Leuconostoc mesenteroides NRRL B-1299
-
 NRRL B-1299
-

Purification (Commentary)

Purification (Comment) Organism
2 forms: I and II Leuconostoc mesenteroides
NRRL B-1299 Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Leuconostoc mesenteroides
250
-
 enzyme II Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (1,6-alpha-D-glucosyl)n
-
 Leuconostoc mesenteroides D-fructose + (1,6-alpha-D-glucosyl)n+1
-
 ?
sucrose + (1,6-alpha-D-glucosyl)n
-
 Leuconostoc mesenteroides NRRL B-1299 D-fructose + (1,6-alpha-D-glucosyl)n+1
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 69000, enzyme I, SDS-PAGE Leuconostoc mesenteroides
monomer 1 * 79000, enzyme II, SDS-PAGE Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 enzyme I is stable for 10 min Leuconostoc mesenteroides
35 40 enzyme II Leuconostoc mesenteroides
45
-
 enzyme I Leuconostoc mesenteroides

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 51 80% of activity maximum at 37°C and 51°C Leuconostoc mesenteroides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 10 min, enzyme I stable Leuconostoc mesenteroides
45
-
 10 min, 80% loss of activity, enzyme I Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6.9 enzyme II Leuconostoc mesenteroides
6.3 6.5 enzyme I Leuconostoc mesenteroides

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.6 7.2 4°C, 24 h, 50% loss of activity at pH 4.6 and 7.2, enzyme II Leuconostoc mesenteroides
4.7 8.5 4°C, 24 h, 50% loss of activity at pH 4.7 and 8.5, enzyme I Leuconostoc mesenteroides
5.3 5.8 4°C, 24 h, enzyme II stable Leuconostoc mesenteroides
6.2 6.9 4°C, 24 h, enzyme I stable Leuconostoc mesenteroides