Cloned (Comment) | Organism |
---|---|
cloning of the full-length CGT cDNA, open reading frame of 1623 bp encodes a 541 amino acids core protein | Rattus norvegicus |
time-regulated CGT expression | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
smooth endoplasmic reticulum | smooth endoplasmic reticulum, with C-terminal endoplasmic reticulum membrane retention signal, C-terminal 20 residue transmembrane domain as anchor of enzyme in the membrane of the ER | Rattus norvegicus | 5790 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
x * 64000, glycosylated protein, x * 59000, deglycosylated protein with glycopeptidase F, SDS-PAGE | Rattus norvegicus |
64000 | - |
x * 64000, glycosylated protein, x * 59000, deglycosylated protein with glycopeptidase F, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | oligodendrocyte-specific enzyme is involved in the biosynthesis of the oligodendrocyte- and myelin-specific cerebrosides, time-regulated CGT expression with a peak around postnatal days 15-25 | ? | - |
? | |
additional information | Rattus norvegicus Wistar | oligodendrocyte-specific enzyme is involved in the biosynthesis of the oligodendrocyte- and myelin-specific cerebrosides, time-regulated CGT expression with a peak around postnatal days 15-25 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Wistar rats | - |
Rattus norvegicus | - |
20 days old | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | high mannose glycoprotein | Rattus norvegicus |
glycoprotein | 3 putative glycosylation sites at Asn-78, Asn-333 and Asn-442, but only Asn-78 and Asn-333 are glycosylated, deglycosylated 59 kDa core protein is still enzymatically active | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
copurified with the L-glutamate/aspartate neurotransmitter transporter GLAST-1 of the central nervous system | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
kidney | only weakly expressed in | Rattus norvegicus | - |
oligodendrocyte | oligodendrocyte-specific enzyme | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | oligodendrocyte-specific enzyme is involved in the biosynthesis of the oligodendrocyte- and myelin-specific cerebrosides, time-regulated CGT expression with a peak around postnatal days 15-25 | Rattus norvegicus | ? | - |
? | |
additional information | oligodendrocyte-specific enzyme is involved in the biosynthesis of the oligodendrocyte- and myelin-specific cerebrosides, time-regulated CGT expression with a peak around postnatal days 15-25 | Rattus norvegicus Wistar | ? | - |
? | |
UDPgalactose + 2-(2-hydroxyacyl)sphingosine | - |
Rattus norvegicus | 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP | cerebroside | ? | |
UDPgalactose + 2-(2-hydroxyacyl)sphingosine | - |
Rattus norvegicus Wistar | 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP | cerebroside | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64000, glycosylated protein, x * 59000, deglycosylated protein with glycopeptidase F, SDS-PAGE | Rattus norvegicus |