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Literature summary for 2.4.1.47 extracted from

  • Schulte, S.; Stoffel, W.
    UDP galactose:ceramide galactosyltransferase and glutamate/aspartate transporter. Copurification, separation and characterization of the two glycoproteins (1995), Eur. J. Biochem., 233, 947-953.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning of the full-length CGT cDNA, open reading frame of 1623 bp encodes a 541 amino acids core protein Rattus norvegicus
time-regulated CGT expression Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Rattus norvegicus
-
-
smooth endoplasmic reticulum smooth endoplasmic reticulum, with C-terminal endoplasmic reticulum membrane retention signal, C-terminal 20 residue transmembrane domain as anchor of enzyme in the membrane of the ER Rattus norvegicus 5790
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
x * 64000, glycosylated protein, x * 59000, deglycosylated protein with glycopeptidase F, SDS-PAGE Rattus norvegicus
64000
-
x * 64000, glycosylated protein, x * 59000, deglycosylated protein with glycopeptidase F, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus oligodendrocyte-specific enzyme is involved in the biosynthesis of the oligodendrocyte- and myelin-specific cerebrosides, time-regulated CGT expression with a peak around postnatal days 15-25 ?
-
?
additional information Rattus norvegicus Wistar oligodendrocyte-specific enzyme is involved in the biosynthesis of the oligodendrocyte- and myelin-specific cerebrosides, time-regulated CGT expression with a peak around postnatal days 15-25 ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
Wistar rats
-
Rattus norvegicus
-
20 days old
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein high mannose glycoprotein Rattus norvegicus
glycoprotein 3 putative glycosylation sites at Asn-78, Asn-333 and Asn-442, but only Asn-78 and Asn-333 are glycosylated, deglycosylated 59 kDa core protein is still enzymatically active Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
copurified with the L-glutamate/aspartate neurotransmitter transporter GLAST-1 of the central nervous system Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-
kidney only weakly expressed in Rattus norvegicus
-
oligodendrocyte oligodendrocyte-specific enzyme Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information oligodendrocyte-specific enzyme is involved in the biosynthesis of the oligodendrocyte- and myelin-specific cerebrosides, time-regulated CGT expression with a peak around postnatal days 15-25 Rattus norvegicus ?
-
?
additional information oligodendrocyte-specific enzyme is involved in the biosynthesis of the oligodendrocyte- and myelin-specific cerebrosides, time-regulated CGT expression with a peak around postnatal days 15-25 Rattus norvegicus Wistar ?
-
?
UDPgalactose + 2-(2-hydroxyacyl)sphingosine
-
Rattus norvegicus 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP cerebroside ?
UDPgalactose + 2-(2-hydroxyacyl)sphingosine
-
Rattus norvegicus Wistar 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP cerebroside ?

Subunits

Subunits Comment Organism
? x * 64000, glycosylated protein, x * 59000, deglycosylated protein with glycopeptidase F, SDS-PAGE Rattus norvegicus