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Literature summary for 2.4.1.47 extracted from
- Brain UDPgalactose: ceramide galactosyltransferase Purification of a catalytically active protein obtained after proteolytic digestion (1976), Biochim. Biophys. Acta, 429, 342-351.
General Stability
| General Stability | Organism |
|---|---|
| glycerol stabilizes | Rattus norvegicus |
| stable in membrane-bound form, activity resisted destruction by pronase treatment at 4°C | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Phospholipase A | almost complete inactivation | Rattus norvegicus | |
| Phospholipase C | 56% loss of activity | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane-bound | Rattus norvegicus | 16020 | - |
| microsome | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 400000 | 500000 | enzyme-phospholipid-detergent complex, gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
15-20 days old | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phospholipoprotein | intact phospholipids required for full activity, high phospholipid content | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 105fold | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.001455 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% w/v glycerol, 1 month, about 30% loss of activity | Rattus norvegicus |
| 0°C, 1 day, 50% loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPgalactose + 2-(2-hydroxyacyl)sphingosine | - |
Rattus norvegicus | 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP | - |
? |  |
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Release 2023.1 (January 2023)
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