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Literature summary for 2.4.1.47 extracted from

  • Neskovic, N.M.; Mandel, P.; Gatt, S.
    UDPgalactose: ceramide galactosyltransferase from rat brain (1981), Methods Enzymol., 71, 521-527.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphatidylcholine stimulates Rattus norvegicus
phosphatidylethanolamine stimulates Rattus norvegicus
Phospholipids addition to incubation mixture containing Triton X-100 increases activity Rattus norvegicus

General Stability

General Stability Organism
glycerol stabilizes Rattus norvegicus
in presence of glycerol the enzyme can be frozen and rethawed several times without appreciable loss of stability Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Detergents inhibition by excess detergent Rattus norvegicus
DL-sphingosine
-
Rattus norvegicus
Octanoyl-D-threo-p-nitro-1-phenyl-2-amino-1,3-propanediol
-
Rattus norvegicus
Protein inhibitor from brain, kidney, spleen and liver of rat and other animals Rattus norvegicus
sodium deoxycholate strong inhibition Rattus norvegicus
sodium taurocholate strong inhibition Rattus norvegicus
sodium taurodeoxycholate strong inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
2-(2-hydroxyacyl)sphingosine Km value for ceramide is influenced by its dispersion state, in presence of 2 mM Triton S-100 biphasic kinetics are observed with increasing ceramide concentration, yielding two apparent Km values of 0.018 and 0.15 mM Rattus norvegicus
0.027 0.034 UDPgalactose
-
Rattus norvegicus
0.15
-
2-(2-hydroxyacyl)sphingosine Km value for ceramide is influenced by its dispersion state, in presence of 2 mM Triton S-100 biphasic kinetics are observed with increasing ceramide concentration, yielding two apparent Km values of 0.018 and 0.15 mM Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
400000 500000 lipoprotein complex Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
15-20 days old
-

Posttranslational Modification

Posttranslational Modification Comment Organism
lipoprotein
-
Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002733
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, 50% w/v glycerol, 1 month, about 30% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: glucosylceramide, lactosylceramide, asialo GM2ganglioside Rattus norvegicus ?
-
?
additional information not: ceramide containing nonhydroxy fatty acids Rattus norvegicus ?
-
?
UDPgalactose + 2-(2-hydroxyacyl)sphingosine i.e. 2-hydroxy fatty acid ceramide Rattus norvegicus 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP cerebroside ?
UDPgalactose + 2-(2-hydroxyacyl)sphingosine i.e. 2-hydroxy fatty acid ceramide Rattus norvegicus 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP 2-hydroxy fatty acid cerebroside ?
UDPgalactose + 2-(2-hydroxyacyl)sphingosine i.e. 2-hydroxy fatty acid ceramide Rattus norvegicus 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP i.e. 2-hydroxy fatty acid galactosylceramide ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.2
-
Rattus norvegicus