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Literature summary for 2.4.1.47 extracted from

  • Basu, S.; Schultz, A.M.; Basu, M.; Roseman, S.
    Enzymatic synthesis of galactocerebroside by a galactosyltransferase from embryonic chicken brain (1971), J. Biol. Chem., 246, 4272-4279.
    View publication on PubMed

General Stability

General Stability Organism
freezing causes 80-85% loss of activity, 2-mercaptoethanol protects against inactivation by freezing Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
DL-sphingosine at higher concentrations Gallus gallus
DL-sphingosine
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
UDPgalactose
-
Gallus gallus
0.11
-
2-(2-hydroxyacyl)sphingosine
-
Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates at 10 mM, dialyzed enzyme has requirement for divalent cations Gallus gallus
Mg2+ stimulates Gallus gallus
Mg2+ stimulates Rattus norvegicus
Mg2+ dialyzed enzyme has requirement for divalent cations, optimal stimulation at 5 mM Gallus gallus
Mn2+ stimulates Gallus gallus
Mn2+ stimulates Rattus norvegicus
Mn2+ at 10 mM, dialyzed enzyme has requirement for divalent cations Gallus gallus
additional information not activated by Cu2+, Fe2+, Co2+, Ni2+, Zn2+, Cd2+ Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPgalactose + 2-(2-hydroxyacyl)sphingosine Gallus gallus i.e. 2-hydroxy fatty acid ceramide, synthesis of cerebroside 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
-
?
UDPgalactose + 2-(2-hydroxyacyl)sphingosine Gallus gallus enzyme may play an important role in the synthesis of at least one type of myelin 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
-
?

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
3 days old
-
Gallus gallus
-
19-20 days old embryos
-
Rattus norvegicus
-
1 month old
-
Sus scrofa
-
fetus
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Gallus gallus
-
brain
-
Cavia porcellus
-
brain
-
Rattus norvegicus
-
brain
-
Sus scrofa
-
embryo maximal activity in 19-20 day-old embryos Gallus gallus
-

Storage Stability

Storage Stability Organism
4°C, dialyzed enzyme, 0.1% 2-mercaptoethanol, 0.32 M sucrose, 7 days, stable Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information
-
Rattus norvegicus ?
-
?
additional information not: galactose, galactose-1-phosphat, ADP-galactose Gallus gallus ?
-
?
UDPgalactose + 2-(2-hydroxyacyl)sphingosine
-
Rattus norvegicus 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP i.e. 2-hydroxy fatty acid galactosylceramide ?
UDPgalactose + 2-(2-hydroxyacyl)sphingosine highly specific Gallus gallus 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP product: galactocerebroside containing 2-hydroxy fatty acids linked to the amino group of the sphingosine moiety ?
UDPgalactose + 2-(2-hydroxyacyl)sphingosine ceramide containing 2-hydroxystearic acid as the predominant fatty acid Gallus gallus 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP product: galactocerebroside containing 2-hydroxy fatty acids linked to the amino group of the sphingosine moiety ?
UDPgalactose + 2-(2-hydroxyacyl)sphingosine i.e. 2-hydroxy fatty acid ceramide, synthesis of cerebroside Gallus gallus 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
-
?
UDPgalactose + 2-(2-hydroxyacyl)sphingosine enzyme may play an important role in the synthesis of at least one type of myelin Gallus gallus 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
-
?
UDPglucose + 2-(2-hydroxyacyl)sphingosine at 7-10% of the activity with UDP-galactose Gallus gallus glucocerebroside + UDP
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Gallus gallus
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
in bicine buffer Gallus gallus