Cloned (Comment) | Organism |
---|---|
cloning, sequencing and expression of wild type and SF32 mutant recombinant pgant35A gene in COS7 cells | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
L195stop | 3775 mutant, more than half of the putative catalytic region is eliminated, early pupal lethality | Drosophila melanogaster |
Q89stop | HG8 mutant, truncated protein within the putative stem region, early pupal lethality | Drosophila melanogaster |
R227W | SF32 mutant with dramatically reduced activity, early pupal lethality | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
UDP-GalNAc | - |
Drosophila melanogaster | |
0.595 | - |
EA2 peptide | - |
Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | type II membrane protein containing a 23-amino acid transmembrane domain and an 8-amino acid cytoplasmic tail | Drosophila melanogaster | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
x * 78000, recombinant wild type and SF32 mutant enzyme, SDS-PAGE | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Drosophila melanogaster | involved in development, necessary for viability | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q8MVS5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, expressed in COS7 cells | Drosophila melanogaster |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | cloning of the pgant35A gene from embryonic cDNA library | Drosophila melanogaster | - |
additional information | expression of enzyme throughout embryonic, larval and pupal stages, in adult males and females, expression increases during larval development and continues through pupal and adult stages | Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | 519-amino acid putative catalytic region | Drosophila melanogaster | ? | - |
? | |
additional information | involved in development, necessary for viability | Drosophila melanogaster | ? | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + EA2 peptide | - |
Drosophila melanogaster | ? | - |
? | |
UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide | acceptor: MUC5AC-13 glycopeptide | Drosophila melanogaster | UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | acceptors: substantial activity only with EA2 peptide, less active with IgAh peptide | Drosophila melanogaster | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | transfer of GalNAc to a serine or threonine residue on the acceptor protein | Drosophila melanogaster | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000, recombinant wild type and SF32 mutant enzyme, SDS-PAGE | Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Drosophila melanogaster |