Cloned (Comment) | Organism |
---|---|
ppGaNTase-T1 is cloned from kidney RNA, amino acid sequence, expression of ppGaNTases as secreted forms in COS7 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D155N | ppGaNTase-T1 mutant with wild type level of enzyme activity, expression in COS7 cells is markedly compromised | Mus musculus |
D156Q | ppGaNTase-T1 mutant without enzyme activity | Mus musculus |
D209A | ppGaNTase-T1 mutant without enzyme activity | Mus musculus |
D209E | ppGaNTase-T1 mutant with very low enzyme activity | Mus musculus |
D209N | ppGaNTase-T1 mutant without enzyme activity | Mus musculus |
D310N | ppGaNTase-T1 mutant with 2% of enzyme activity | Mus musculus |
D375A | ppGaNTase-T1 mutant with little effect on enzyme activity | Mus musculus |
D375N | ppGaNTase-T1 mutant with little effect on enzyme activity | Mus musculus |
E127Q | ppGaNTase-T1 mutant with less than 1% of enzyme activity | Mus musculus |
E150Q | ppGaNTase-T1 mutant with wild type level of enzyme activity | Mus musculus |
E213Q | ppGaNTase-T1 mutant with less than 1% of enzyme activity | Mus musculus |
E319Q | ppGaNTase-T1 mutant without enzyme activity | Mus musculus |
E322Q | ppGaNTase-T1 mutant with 1% of enzyme activity | Mus musculus |
E376Q | ppGaNTase-T1 mutant with little effect on enzyme activity | Mus musculus |
H125F | active ppGaNTase-T1 mutant, near 3fold greater activity than wild type enzyme | Mus musculus |
H125Q | active ppGaNTase-T1 mutant | Mus musculus |
H211D | ppGaNTase-T1 mutant without enzyme activity | Mus musculus |
H341A | ppGaNTase-T1 mutant with little effect on enzyme activity | Mus musculus |
H341K | ppGaNTase-T1 mutant with little effect on enzyme activity | Mus musculus |
H341L | ppGaNTase-T1 mutant with little effect on enzyme activity | Mus musculus |
H341R | ppGaNTase-T1 mutant with little effect on enzyme activity | Mus musculus |
H341V | ppGaNTase-T1 mutant with little effect on enzyme activity | Mus musculus |
additional information | mutations in the C-terminal 128-amino acid ricin-like lectin motif of ppGaNTase-T1 do not alter its catalytic properties | Mus musculus |
N320A | ppGaNTase-T1 mutant with little effect on enzyme activity | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Mus musculus | 5794 | - |
membrane | type II membrane protein | Mus musculus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | requirement | Mus musculus | |
additional information | metal-dependent enzyme | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + polypeptide | Mus musculus | initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Mus musculus |
recombinant ppGaNTases expressed in COS7 cells | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme structure, GT1 motif, DXD motif, Gal/GalNAc-T motif | Mus musculus | ? | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | ppGaNTase-T1: EA2 peptide acceptor, interdependence of binding of UDP-GalNAc and acceptor substrate, enzyme undergoes a conformational change upon sugar binding, equilibrium between open and closed states may be used as a regulation mechanism of activity | Mus musculus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? | |
UDP-N-acetyl-D-galactosamine + polypeptide | initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function | Mus musculus | UDP + N-acetyl-D-galactosaminyl-polypeptide | - |
? |