Crystallization (Comment) | Organism |
---|---|
structures of GTA, GTB and several chimeras determined by single-crystal X-ray diffraction demonstrate a range of susceptibility to the choice of cryoprotectant, in which the mobile polypeptide loops can be induced by glycerol to form the ordered closed conformation associated with substrate recognition and by MPD (hexylene glycol, 2-methyl-2,4-pentanediol) to hinder binding of substrate in the active site owing to chelation of the Mn2+ cofactor and thereby adopt the disordered open state | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | chimeric GTA and GTB enzymes are generated (AABB, ABBA and ABBB) | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | cofactor | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | galactose is used as an acceptor analogue and UDP as a donor analogue in all soaking trials | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
blood group A glycosyltransferase | - |
Homo sapiens |
GTA | - |
Homo sapiens |