Literature summary for 2.4.1.4 extracted from

Remaud-Simeon, M.; Albaret, F.; Canard, B.; Varlet, I.; Colonna, P.; Willemot, R.M.; Monsan, P.
Studies on a recombinant amylosucrase (1995), Prog. Biotechnol., 10, 313-320.

No PubMed abstract available

Search for more literature for 2.4.1.4

Activating Compound

Activating Compound	Comment	Organism	Structure
glycogen	activates recombinant enzyme	Neisseria polysaccharea
maltooligosaccharides	activate recombinant enzyme	Neisseria polysaccharea
starch	activates recombinant enzyme	Neisseria polysaccharea

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Neisseria polysaccharea

Organism

Organism	UniProt	Comment	Textmining
Neisseria polysaccharea	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Neisseria polysaccharea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
9.57	-	recombinant enzyme	Neisseria polysaccharea

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + (1,4-alpha-D-glucosyl)n	-	Neisseria polysaccharea	D-fructose + (1,4-alpha-D-glucosyl)n+1	recombinant enzyme produces glucopolysaccharide mainly composed of alpha-(1-4) glucosidic linkages and a very low degree, i.e. less than 5%, of alpha-(1-6) branched linkages	?
sucrose + maltopentaose	-	Neisseria polysaccharea	D-fructose + maltohexaose + maltoheptaose	-	?

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Release 2023.1 (January 2023)