Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target enzyme for drug design | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,6-dithio-N-butyryl-GlcNbeta-(2-naphthyl) | 45% inhibition at 1.0 mM | Bos taurus | |
1-thio-N-butyryl-GlcNbeta-(2-naphthyl) | uncompetitive, complete inhibition at 1.0 mM, 85% inhibition at 0.2 mM | Bos taurus | |
1-thioGlcNAcbeta-(2-naphthyl) | 91% inhibition at 1.0 mM | Bos taurus | |
6-thio-N-butyryl-GlcNbeta-(2-naphthyl) | 19% inhibition at 1.0 mM | Bos taurus | |
GlcNAcbeta-(2-naphthyl) | 92% inhibition at 1.0 mM | Bos taurus | |
additional information | inhibitor synthesis and potency, mechanism of inhibition, overview | Bos taurus | |
N-butyryl-GlcNbeta-(2-naphthyl) | 87% inhibition at 1.0 mM | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme catalyzes the transfer of Gal from UDP-Gal to GlcNAc-terminating acceptors with inversion of configuration of the glycosidic linkage, in presence of beta-lactalbumin, the enzyme changes its specificity to become lactose synthase, EC 2.4.1.22, and transfers Gal to glucose to synthesize lactose, substrate specificity, e.g. with GlcNAc-terminating O-glycopeptides, no activity with N-trimethylacetyl-GlcN, N,N-dimethyl-GlcN, N-isopropyl-GlcN, and N-(4-MeOBn)GlcN, overview | Bos taurus | ? | - |
? | |
UDP-galactose + 3-deoxy-3-fluoro-GlcNAcbeta-Bn | - |
Bos taurus | UDP + Galbeta(1-4)3-deoxy-3-fluoro-GlcNAcbeta-Bn | - |
? | |
UDP-galactose + 6-deoxy-GlcNAcbeta-Bn | low activity | Bos taurus | UDP + Galbeta(1-4)6-deoxy-GlcNAcbeta-Bn | - |
? | |
UDP-galactose + 6-thio-GlcNAcbeta-Bn | low activity | Bos taurus | UDP + Galbeta(1-4)6-thio-GlcNAcbeta-Bn | - |
? | |
UDP-galactose + GlcNAcbeta-Bn | - |
Bos taurus | UDP + Galbeta(1-4)GlcNAcbeta-Bn | - |
? | |
UDP-galactose + N-4-toluenesulfonyl-GlcN | - |
Bos taurus | UDP + Galbeta(1-4)N-4-toluenesulfonyl-GlcN | - |
? | |
UDP-galactose + N-acetylglucosamine | - |
Bos taurus | UDP + N-acetyllactosamine | - |
? | |
UDP-galactose + N-butyryl-GlcNbeta-Bn | - |
Bos taurus | UDP + Galbeta(1-4)N-butyryl-GlcNbeta-Bn | - |
? | |
UDP-galactose + N-methanesulfonyl-GlcN | - |
Bos taurus | UDP + Galbeta(1-4)N-methanesulfonyl-GlcN | - |
? | |
UDP-galactose + N-trifluoroacetyl-GlcN | - |
Bos taurus | UDP + Galbeta(1-4)N-trifluoroacetyl-GlcN | - |
? | |
UDP-galactose + T(GlcNAcbeta3GalNAcbeta)TTVTPTPTG | - |
Bos taurus | ? | - |
? | |
UDP-galactose + T(GlcNAcbeta6GalNAcbeta)TTVTPTPTG | - |
Bos taurus | ? | - |
? | |
UDP-galactose + T(GlcNAcbeta6[GlcNAcbeta3]GalNAcbeta)TTVTPTPTG | - |
Bos taurus | ? | - |
? | |
UDP-galactose + TT(GlcNAcbeta3GalNAcbeta)TVTPTPTG | - |
Bos taurus | ? | - |
? | |
UDP-galactose + TT(GlcNAcbeta6GalNAcbeta)TVTPTPTG | - |
Bos taurus | ? | - |
? | |
UDP-galactose + TT(GlcNAcbeta6[GlcNAcbeta3]GalNAcbeta)TVTPTPTG | - |
Bos taurus | ? | - |
? | |
UDP-galactose + TTTV (GlcNAcbeta3GalNAcbeta)TPTPTG | - |
Bos taurus | ? | - |
? | |
UDP-galactose + TTTV(GlcNAcbeta6GalNAcbeta)TPTPTG | - |
Bos taurus | ? | - |
? | |
UDP-galactose + TTTV(GlcNAcbeta6[GlcNAcbeta3]GalNAcbeta)TPTPTG | - |
Bos taurus | ? | - |
? | |
UDP-galactose + TTTVTP(GlcNAcbeta3GalNAcbeta)TPTG | - |
Bos taurus | ? | - |
? | |
UDP-galactose + TTTVTP(GlcNAcbeta6GalNAcbeta)TPTG | - |
Bos taurus | ? | - |
? | |
UDP-galactose + TTTVTPTP(GlcNAcbeta3GalNAcbeta)TG | - |
Bos taurus | ? | - |
? | |
UDP-galactose + TTTVTPTP(GlcNAcbeta6[GlcNAcbeta3]GalNAcbeta)TG | - |
Bos taurus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta1,4-Gal-transferase T1 | - |
Bos taurus |
beta4GalT | - |
Bos taurus |
More | cf. EC 2.4.1.90, the enzyme belongs to the CAZY family 7 of inverting glycosyltransferases | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Bos taurus | |
0.01 | - |
1-thio-N-butyryl-GlcNbeta-(2-naphthyl) | pH 7.0 | Bos taurus |