Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.37 extracted from

  • Gagnon, S.; Legg, M.; Polakowski, R.; Letts, J.; Persson, M.; Lin, S.; Zheng, R.; Rempel, B.; Schuman, B.; Haji-Ghassemi, O.; Borisova, S.; Palcic, M.; Evans, S.
    Conserved residues Arg188 and Asp302 are critical for active site organization and catalysis in human ABO(H) blood group A and B glycosyltransferases (2018), Glycobiology, 28, 624-636 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.37

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of wild-type and mutants D302A, D302C, D302L, R188K. Conserved active site residues Arg188 and Asp302 are critical for catalysis, and disruption of their hydrogen bond network through mutation can dramatically decrease enzymatic activity Homo sapiens

Protein Variants

Protein Variants Comment Organism
D302A almost complete loss of activity Homo sapiens
D302A/D316A strong decrease in kcat value Homo sapiens
D302C kcat value is 9% that of wild-type GTB Homo sapiens
D302E kcat value is 47% that of wild-type GTB Homo sapiens
D302E/D316E 50% decrease in kcat value Homo sapiens
D302L almost complete loss of activity Homo sapiens
R188K almost complete loss of activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
 UDP-alpha-D-galactose wild-type, pH 7.0, 37°C Homo sapiens
0.053
-
 UDP-alpha-D-galactose mutant D302C, pH 7.0, 37°C Homo sapiens
0.088
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 wild-type, pH 7.0, 37°C Homo sapiens
0.1
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant D302E/D316E, pH 7.0, 37°C Homo sapiens
0.101
-
 UDP-alpha-D-galactose mutant R188K, pH 7.0, 37°C Homo sapiens
0.101
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant R188K, pH 7.0, 37°C Homo sapiens
0.112
-
 UDP-alpha-D-galactose mutant D302E/D316E, pH 7.0, 37°C Homo sapiens
0.117
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant D302A/D316A, pH 7.0, 37°C Homo sapiens
0.17
-
 UDP-alpha-D-galactose mutant D302A/D316A, pH 7.0, 37°C Homo sapiens
0.332
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant D302C, pH 7.0, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P16422
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-galactose + alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3
-
 Homo sapiens UDP + alpha-D-Gal-(1->3)-[alpha-Fuc-(1->2)]-beta-D-Gal-(CH2)7CH3
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0006
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant R188K, pH 7.0, 37°C Homo sapiens
0.002
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant D302A/D316A, pH 7.0, 37°C Homo sapiens
0.46
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant D302C, pH 7.0, 37°C Homo sapiens
2.4
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 mutant D302E/D316E, pH 7.0, 37°C Homo sapiens
5.1
-
 alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 wild-type, pH 7.0, 37°C Homo sapiens