Cloned (Comment) | Organism |
---|---|
overexpression of truncated GTB in Escherichia coli strain BL21 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of truncated GTB monomers composed of the full C-terminal and catalytic domain as well as a truncated N-terminal domain | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic mechanism and thermodynamic analysis of GTB using electrospray ionization mass spectrometry, comparison to GTA, EC 2.4.1.40 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required, in the absence of divalent metal ion, GTB does not exhibit any appreciable affinity for its native donor UDP-Gal | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34483 | - |
x * 34483, recombinant truncated enzyme mutant, mass spectrometry | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactose | Homo sapiens | - |
UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactose | - |
? | |
UDP-galactose + blood group antigen H | Homo sapiens | - |
UDP + alpha-D-galactosyl-blood group antigen H | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme-substrate interaction analysis by electrospray ionization mass spectrometry, binding of substrate and product analogues, overview | Homo sapiens | ? | - |
? | |
UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactose | - |
Homo sapiens | UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactose | - |
? | |
UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactose | i.e. H disaccharide | Homo sapiens | UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactose | in the absence of UDP and Mn2+, GTB recognizes its trisaccharide product with a low affinity of about 1 mM, while the presence of UDP and Mn2+ precludes B-trisaccharide binding | ? | |
UDP-galactose + blood group antigen H | - |
Homo sapiens | UDP + alpha-D-galactosyl-blood group antigen H | - |
? | |
UDP-galactose + blood group antigen H | GTB catalyzes the transfer of galactose from UDP-Gal to the C3 position of the terminal galactose of H antigen acceptors | Homo sapiens | UDP + alpha-D-galactosyl-blood group antigen H | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34483, recombinant truncated enzyme mutant, mass spectrometry | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
alpha-(1,3)-galactosyltransferase | - |
Homo sapiens |
GTB | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
assay at | Homo sapiens |