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Literature summary for 2.4.1.37 extracted from
- A high-throughput pH indicator assay for screening glycosyltransferase saturation mutagenesis libraries (2008), Anal. Biochem., 378, 1-7.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| M214G | saturation mutagenesis of GTB enzyme at M214 leads to a two-fold higher kcat for UDP-GalNAc and specific activity of the mutant compared to the wild-type GTB | Homo sapiens |
| M214S | saturation mutagenesis of GTB enzyme at M214 leads to a two-fold higher kcat for UDP-GalNAc and specific activity of the mutant compared to the wild-type GTB | Homo sapiens |
| additional information | screening of a saturation mutagenesis library for mutant determination with altered cosubstrate specificity | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell surface | - |
Homo sapiens | 9986 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactosyl-R | Homo sapiens | - |
UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactosyl-R | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.05 | - |
wild-type enzyme, with cosubstrate UDP-GalNAc | Homo sapiens |
| 0.11 | - |
mutant M214G, with cosubstrate UDP-GalNAc | Homo sapiens |
| 0.14 | - |
mutant M214S, with cosubstrate UDP-GalNAc | Homo sapiens |
| 1.9 | - |
mutant M214S, with cosubstrate UDP-Gal | Homo sapiens |
| 3.3 | - |
wild-type enzyme, with cosubstrate UDP-Gal | Homo sapiens |
| 4.4 | - |
mutant M214G, with cosubstrate UDP-Gal | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | development and evaluation of a general screening assay method for glycosyltransferase activities, overview | Homo sapiens | ? | - |
? | |
| UDP-galactose + alpha-L-fucosyl-(1,2)-beta-D-galactosyl-O-(CH2)7CH3 | i.e. alpha-L-Fucp-(1,2)-beta-DGalp-O-(CH2)7CH3 | Homo sapiens | UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl-(1,2)]-beta-D-galactosyl-O-(CH2)7CH3 | - |
? | |
| UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactosyl-R | - |
Homo sapiens | UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactosyl-R | - |
? | |
| UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactosyl-R | importance of residue M214 for donor enzyme specificity | Homo sapiens | UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactosyl-R | - |
? | |
| UDP-galactose + L-2'-L-fucosyllactose | - |
Homo sapiens | UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactosyl-D-glucose | - |
? | |
| UDP-N-acetylgalactosamine + L-2'-fucosyllactose | - |
Homo sapiens | UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactosyl-D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| blood group alpha-(1-3)-galactosyltransferase | - |
Homo sapiens |
| GTB | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.37 | - |
UDP-Gal | pH 7.0, 37°C, recombinant wild-type enzyme | Homo sapiens | |
| 0.7 | - |
UDP-Gal | pH 7.0, 37°C, recombinant mutant M214S | Homo sapiens | |
| 0.74 | - |
UDP-Gal | pH 7.0, 37°C, recombinant mutant M214G | Homo sapiens | |
| 4 | - |
UDP-GalNAc | pH 7.0, 37°C, recombinant mutant M214G | Homo sapiens | |
| 5 | - |
UDP-GalNAc | pH 7.0, 37°C, recombinant mutant M214S | Homo sapiens | |
| 5.1 | - |
UDP-GalNAc | pH 7.0, 37°C, recombinant wild-type enzyme | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.3 | assay at | Homo sapiens |
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