Literature summary for 2.4.1.37 extracted from

Marcus, S.L.; Polakowski, R.; Seto, N.O.L.; Leinala, E.; Borisova, S.; Blancher, A.; Roubinet, F.; Evans, S.V.; Palcic, M.M.
A single point mutation reverses the donor specificity of human blood group B-synthesizing galactosyltransferase (2003), J. Biol. Chem., 278, 12403-12405.

Search for more literature for 2.4.1.37

Cloned(Commentary)

Cloned (Comment)	Organism
P234S-mutant expressed in Escherichia coli BL21	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
P234S-mutant, 1.55 and 1.65 A resolution, with and without H-antigen	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
P234S	dramatic and complete reversal of donor specificity, it preferentially utilizes UDP-GalNac for transfer	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.027	-	L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3	-	Homo sapiens
0.088	-	UDP-galactose	-	Homo sapiens
0.106	-	L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3	P234S mutant	Homo sapiens
4.5	-	UDP-galactose	P234S mutant	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P16442	only four amino acids are responsible for B and A blood group enzymes R176G, G235S, L266M and G268A, the latter two amino acids are responsible for the difference in donor specificity and the first two have roles in acceptor binding and turnover	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-galactose + glycoprotein alpha-L-fucosyl-1,2-D-galactose	-	Homo sapiens	UDP + glycoprotein alpha-D-galactosyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose	-	?
UDP-galactose + L-fucosyl-alpha-1,2-beta-D-galactosyl-O(CH2)7CH3	-	Homo sapiens	UDP + alpha-D-galactosyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-galactosyl-O(CH2)7CH3	-	?

Synonyms

Synonyms	Comment	Organism
GTB	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.004	-	UDP-galactose	P234S mutant	Homo sapiens
0.085	-	UDP-galactose	-	Homo sapiens

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Release 2023.1 (January 2023)