Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli with N-terminal T7 and C-terminal His6 tags | Methanococcus voltae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the enzyme is purified from membrane fraction of recombinant Escherichia coli | Methanococcus voltae | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37328 | - |
x * 37328, calculated from sequence | Methanococcus voltae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate | Methanococcus voltae | the enzyme participates in the N-linked glycosylation in Methanococcus voltae. Methanococcus voltae generates N-linked glycoproteins with a unique trisaccharide composed of ManNAc(6Thr)A-beta1,4-Glc-2,3-diNAcA-beta1,3-GlcNAc in which threonine is linked via an amide to the terminal mannuronic acid | UDP + dolichyl 3-O-(2,3-diacetamido-2,3-dideoxy-beta-D-glucuronsyl)-N-acetyl-alpha-D-glucosaminyl phosphate | - |
? | |
UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate | Methanococcus voltae PS | the enzyme participates in the N-linked glycosylation in Methanococcus voltae. Methanococcus voltae generates N-linked glycoproteins with a unique trisaccharide composed of ManNAc(6Thr)A-beta1,4-Glc-2,3-diNAcA-beta1,3-GlcNAc in which threonine is linked via an amide to the terminal mannuronic acid | UDP + dolichyl 3-O-(2,3-diacetamido-2,3-dideoxy-beta-D-glucuronsyl)-N-acetyl-alpha-D-glucosaminyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanococcus voltae | B3VA59 | - |
- |
Methanococcus voltae PS | B3VA59 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanococcus voltae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate | the enzyme participates in the N-linked glycosylation in Methanococcus voltae. Methanococcus voltae generates N-linked glycoproteins with a unique trisaccharide composed of ManNAc(6Thr)A-beta1,4-Glc-2,3-diNAcA-beta1,3-GlcNAc in which threonine is linked via an amide to the terminal mannuronic acid | Methanococcus voltae | UDP + dolichyl 3-O-(2,3-diacetamido-2,3-dideoxy-beta-D-glucuronsyl)-N-acetyl-alpha-D-glucosaminyl phosphate | - |
? | |
UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate | the enzyme is specific for dolichyl N-acetyl-alpha-D-glucosaminyl phosphate, as no conversion is observed with Dol-P or Dol-PP-GlcNAc. No activity with UDP-Glc, UDP-GalNAc or UDP-Gal. Although the full length N-glycan generated by Methanococcus voltae is a trisaccharide (composed of ManNAc(6Thr)A-beta1,4-Glc-2,3-diNAcA-beta1,3-GlcNAc in which threonine is linked via an amide to the terminal mannuronic acid) the disaccharide-linked | Methanococcus voltae | UDP + dolichyl 3-O-(2,3-diacetamido-2,3-dideoxy-beta-D-glucuronsyl)-N-acetyl-alpha-D-glucosaminyl phosphate | - |
? | |
UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate | the enzyme participates in the N-linked glycosylation in Methanococcus voltae. Methanococcus voltae generates N-linked glycoproteins with a unique trisaccharide composed of ManNAc(6Thr)A-beta1,4-Glc-2,3-diNAcA-beta1,3-GlcNAc in which threonine is linked via an amide to the terminal mannuronic acid | Methanococcus voltae PS | UDP + dolichyl 3-O-(2,3-diacetamido-2,3-dideoxy-beta-D-glucuronsyl)-N-acetyl-alpha-D-glucosaminyl phosphate | - |
? | |
UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate | the enzyme is specific for dolichyl N-acetyl-alpha-D-glucosaminyl phosphate, as no conversion is observed with Dol-P or Dol-PP-GlcNAc. No activity with UDP-Glc, UDP-GalNAc or UDP-Gal. Although the full length N-glycan generated by Methanococcus voltae is a trisaccharide (composed of ManNAc(6Thr)A-beta1,4-Glc-2,3-diNAcA-beta1,3-GlcNAc in which threonine is linked via an amide to the terminal mannuronic acid) the disaccharide-linked | Methanococcus voltae PS | UDP + dolichyl 3-O-(2,3-diacetamido-2,3-dideoxy-beta-D-glucuronsyl)-N-acetyl-alpha-D-glucosaminyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37328, calculated from sequence | Methanococcus voltae |
Synonyms | Comment | Organism |
---|---|---|
AglC | - |
Methanococcus voltae |
Mv990 | locus name | Methanococcus voltae |
UDP-Glc-2,3-diNAcA glycosyltransferase | - |
Methanococcus voltae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Methanococcus voltae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Methanococcus voltae |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme participates in the N-linked glycosylation in Methanococcus voltae. Methanococcus voltae generates N-linked glycoproteins with a unique trisaccharide composed of ManNAc(6Thr)A-beta1,4-Glc-2,3-diNAcA-beta1,3-GlcNAc in which threonine is linked via an amide to the terminal mannuronic acid | Methanococcus voltae |