Application | Comment | Organism |
---|---|---|
synthesis | an engineered strain of Neurospora crassa (F5) with six of seven beta-glucosidase (bgl) genes knocked out produces cellobiose and cellobionate directly from cellulose without the addition of exogenous cellulases. Further modification by knock-out of catabolite repression genes, cre-1 and ace-1, leads to improved cellobiose dehydrogenase and exoglucanase expression but not to an improvement in cellobiose or cellobionate production. Deletion of the cellobionate phosphorylase gene NdvB from the genome of F5 lacking ace-1 and cre-1 to prevent the consumption of cellobiose and cellobionate results in a slightly reduced hydrolysis rate and in convertsion of 75% of the cellulose consumed to the desired products, cellobiose and cellobionate, compared to 18% converted by the strain F5 lackong only ace-1 cre-1 | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | Q7S0S2 | - |
- |
Neurospora crassa DSM 1257 | Q7S0S2 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
NdvB | - |
Neurospora crassa |