Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.32 extracted from

  • Piro, G.; Zuppa, A.; Dalessandro, G.; Northcote, D.H.
    Glucomannan synthesis in pea epicotyls: the mannose and glucose transferases (1993), Planta, 190, 206-220.
    View publication on PubMed
Search for more literature for 2.4.1.32

Activating Compound

Activating Compound Comment Organism Structure
additional information not affected by spermine, spermidine, cellobiose, tunicamycin, bacitracin, EDTA and EGTA Pisum sativum

Inhibitors

Inhibitors Comment Organism Structure
ATP slight Pisum sativum
Ca2+ above 5 mM Pisum sativum
CHAPS 0.25-1% Pisum sativum
CHAPSO 0.25-1% Pisum sativum
Co2+ above 5 mM Pisum sativum
CTP slight Pisum sativum
cyclohexandione slight inactivation Pisum sativum
Digitonin slightly inhibitory at 1% Pisum sativum
formaldehyde 46% inactivation at 10 mM in presence of NaCNBH3 Pisum sativum
GDP
-
 Pisum sativum
GDP-D-glucose competitive Pisum sativum
GDP-L-fucose 40% inhibition at 1 mM Pisum sativum
glycerol slightly above 5% Pisum sativum
GMP
-
 Pisum sativum
GTP
-
 Pisum sativum
Mg2+ slightly inhibitory at 1-15 mM Pisum sativum
Mn2+ strong inhibition at 5 mM Pisum sativum
additional information not affected by N-acetylimidazol, acetyl-CoA, spermine, spermidine, cellobiose, tunicamycin, bacitracin, EDTA and EGTA Pisum sativum
N-ethylmaleimide concentration-dependent inactivation Pisum sativum
Phenylglyoxal concentration and time-dependent Pisum sativum
Phospholipase A2 fast inactivation, irreversible Pisum sativum
Phospholipase C slow inactivation, irreversible Pisum sativum
Triton X-100 0.25-1% Pisum sativum
UDP slight Pisum sativum
UTP slight Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 GDP-D-mannose
-
 Pisum sativum
0.182
-
 GDP-D-mannose digitonin-solubilized enzyme Pisum sativum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Pisum sativum 16020
-

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
 var. Alaska
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling stem segment of third internode of etiolated seedling Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 kinetics Pisum sativum
0.0017
-
 membrane-bound and digitonin-solubilized enzyme Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-D-mannose + (glucomannan)n glucomannan is soluble in 10% trichloroacetic acid and in water Pisum sativum GDP + (glucomannan)n+1 mannose attached by beta-1,4-linkages ?
GDP-D-mannose + (glucomannan)n glucomannan is soluble in 10% trichloroacetic acid and in water Pisum sativum GDP + (glucomannan)n+1 product analysis ?
GDP-D-mannose + (glucomannan)n alkali insoluble glucomannan Pisum sativum GDP + (glucomannan)n+1 mannose attached by beta-1,4-linkages ?
GDP-D-mannose + (glucomannan)n alkali insoluble glucomannan Pisum sativum GDP + (glucomannan)n+1 product analysis ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Pisum sativum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 30% remaining activity at 2°C Pisum sativum
additional information
-
 cold-stress does not alter enzyme activity Pisum sativum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.001
-
 GDP-D-glucose digitonin-solubilized enzyme Pisum sativum
0.006
-
 GDP-D-glucose
-
 Pisum sativum