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Literature summary for 2.4.1.302 extracted from

  • Pi, N.; Meyers, C.L.; Pacholec, M.; Walsh, C.T.; Leary, J.A.
    Mass spectrometric characterization of a three-enzyme tandem reaction for assembly and modification of the novobiocin skeleton (2004), Proc. Natl. Acad. Sci. USA, 101, 10036-10041.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.302

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0086
-
 N-(4,7-dihydroxy-8-methyl-2-oxo-2H-chromen-3-yl)-4-hydroxy-3-methylbenzamide pH 6.0, temperature not specified in the publication Streptomyces sp.
0.039
-
 novobiocic acid pH 6.0, temperature not specified in the publication Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-beta-L-noviose + novobiocic acid Streptomyces sp.
-
 dTDP + demethyldecarbamoyl novobiocin i.e. demethyldecarbamoyl novobiocin ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-beta-L-noviose + N-(4,7-dihydroxy-8-methyl-2-oxo-2H-chromen-3-yl)-4-hydroxy-3-methylbenzamide
-
 Streptomyces sp. dTDP + N-[7-[(6-deoxy-5-methyl-beta-D-gulopyranosyl)oxy]-4-hydroxy-8-methyl-2-oxo-2H-chromen-3-yl]-4-hydroxy-3-methylbenzamide
-
 ?
dTDP-beta-L-noviose + novobiocic acid
-
 Streptomyces sp. dTDP + demethyldecarbamoyl novobiocin i.e. demethyldecarbamoyl novobiocin ?
dTDP-beta-L-noviose + novobiocic acid sequential bi-bi catalytic mechanism. NovM binds to both donor and acceptor substrate (novobiocic acid and dTDP-L-noviose) to form a ternary complex, during which process the noviose sugar is transferred from dTDP-L-noviose to novobiocic acid. The glycosylated product, demethyldecarbamoyl novobiocin, is subsequently released from the complex Streptomyces sp. dTDP + demethyldecarbamoyl novobiocin i.e. demethyldecarbamoyl novobiocin ?

Synonyms

Synonyms Comment Organism
NovM
-
 Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
219
-
 novobiocic acid pH 6.0, temperature not specified in the publication Streptomyces sp.
290
-
 N-(4,7-dihydroxy-8-methyl-2-oxo-2H-chromen-3-yl)-4-hydroxy-3-methylbenzamide pH 6.0, temperature not specified in the publication Streptomyces sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3300
-
 N-(4,7-dihydroxy-8-methyl-2-oxo-2H-chromen-3-yl)-4-hydroxy-3-methylbenzamide pH 6.0, temperature not specified in the publication Streptomyces sp.
5615
-
 novobiocic acid pH 6.0, temperature not specified in the publication Streptomyces sp.