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Literature summary for 2.4.1.296 extracted from

  • Jonsson, L.M.V.; Aarsman, M.E.G.; van Diepen, J.; de Vlaming, P.; Smit, N.; Schram, A.W.
    Properties and genetic control of anthocyanin 5-O-glucosyltransferase in flowers of Petunia hybrida (1984), Planta, 160, 341-347.
    View publication on PubMed
Search for more literature for 2.4.1.296

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by EDTA at 5 mM Petunia x hybrida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 delphinidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] pH 8.3, 30°C Petunia x hybrida
0.003
-
 petunidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] pH 8.3, 30°C Petunia x hybrida
0.22
-
 UDP-alpha-D-glucose pH 8.3, 30°C Petunia x hybrida

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no stimulation by 1 mM Mg2+ or Ca2+ Petunia x hybrida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 x * 52000, SDS-PAGE Petunia x hybrida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + delphinidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] Petunia x hybrida i.e. delphinidin 3-(4-coumaroyl)-rutinoside UDP + delphinidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] 5-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + petunidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] Petunia x hybrida i.e. petunidin 3-(4-coumaroyl)-rutinoside UDP + petunidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] 5-O-beta-D-glucoside
-
 ?

Organism

Organism UniProt Comment Textmining
Petunia x hybrida
-
-
-
Petunia x hybrida
-
 gene gene Anl
-

Purification (Commentary)

Purification (Comment) Organism
30fold from flowers, by ammonium sulfate fractionation, desalting gel filtration, separation of the 5-O-glucosyltransferase from the anthocyanidin 3-O-glucosyltransferase by chromatofocusing, followed by ultrafiltration Petunia x hybrida

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Petunia x hybrida
-
pollen
-
 Petunia x hybrida
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.05
-
 purified native enzyme, pH 8.3, 30°C Petunia x hybrida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity, overview. No activity with delphinidin 3-rutinoside, cyanidin 3-rutinoside, and delphinidin 3-glucoside Petunia x hybrida ?
-
 ?
UDP-alpha-D-glucose + delphinidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] i.e. delphinidin 3-(4-coumaroyl)-rutinoside Petunia x hybrida UDP + delphinidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] 5-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + petunidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] i.e. petunidin 3-(4-coumaroyl)-rutinoside Petunia x hybrida UDP + petunidin 3-O-[2-O-(4-coumaroyl)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside] 5-O-beta-D-glucoside
-
 ?

Subunits

Subunits Comment Organism
? x * 52000, SDS-PAGE Petunia x hybrida

Synonyms

Synonyms Comment Organism
anthocyanin 5-O-glucosyltransferase
-
 Petunia x hybrida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Petunia x hybrida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
 Petunia x hybrida

pI Value

Organism Comment pI Value Maximum pI Value
Petunia x hybrida chromatofocusing
-
 4.75