Cloned (Comment) | Organism |
---|---|
PH1, cloning by hybridization screening, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression in Saccharomyces cerevisiae strain G1315 | Petunia x hybrida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52163 | - |
x * 52163, PH1, sequence calculation | Petunia x hybrida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + delphinidin 3-O-(4-coumaroyl)-rutinoside | Petunia x hybrida | PH1 represents a 5-GT that exhibits a strict substrate specificity towards its biosynthetically proper substrate, delphinidin 3-(4-coumaroyl)-rutinoside | UDP + delphinidin 3-O-(4-coumaroyl)-rutinoside 5-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + delphinidin 3-O-(4-coumaroyl)-rutinoside | Petunia x hybrida Surfinia Purple | PH1 represents a 5-GT that exhibits a strict substrate specificity towards its biosynthetically proper substrate, delphinidin 3-(4-coumaroyl)-rutinoside | UDP + delphinidin 3-O-(4-coumaroyl)-rutinoside 5-O-beta-D-glucoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petunia x hybrida | - |
two copies gene | - |
Petunia x hybrida Surfinia Purple | - |
two copies gene | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Petunia x hybrida | - |
flower bud | - |
Petunia x hybrida | - |
additional information | mRNA expression of 5-GT increases in the early developmental stages of the flower, reaching the maximum at the stage before flower opening | Petunia x hybrida | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the recombinant PH1 protein exhibited a strict substrate specificity towards anthocyanidin 3-acylrutinoside. No activity with delphinidin 3-rutinoside, cyanidin 3-O-rutinoside, delphinidin 3-O-glucoside, pelargonidin 3-O-glucoside, cyanidin 3-O-glucoside, cyanidin 3-O-caffeoyl-glucoside, and with cyanidin 3,5-diglucoside and cyanidin | Petunia x hybrida | ? | - |
? | |
additional information | the recombinant PH1 protein exhibited a strict substrate specificity towards anthocyanidin 3-acylrutinoside. No activity with delphinidin 3-rutinoside, cyanidin 3-O-rutinoside, delphinidin 3-O-glucoside, pelargonidin 3-O-glucoside, cyanidin 3-O-glucoside, cyanidin 3-O-caffeoyl-glucoside, and with cyanidin 3,5-diglucoside and cyanidin | Petunia x hybrida Surfinia Purple | ? | - |
? | |
UDP-alpha-D-glucose + delphinidin 3-O-(4-coumaroyl)-rutinoside | - |
Petunia x hybrida | UDP + delphinidin 3-O-(4-coumaroyl)-rutinoside 5-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + delphinidin 3-O-(4-coumaroyl)-rutinoside | PH1 represents a 5-GT that exhibits a strict substrate specificity towards its biosynthetically proper substrate, delphinidin 3-(4-coumaroyl)-rutinoside | Petunia x hybrida | UDP + delphinidin 3-O-(4-coumaroyl)-rutinoside 5-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + delphinidin 3-O-(4-coumaroyl)-rutinoside | - |
Petunia x hybrida Surfinia Purple | UDP + delphinidin 3-O-(4-coumaroyl)-rutinoside 5-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + delphinidin 3-O-(4-coumaroyl)-rutinoside | PH1 represents a 5-GT that exhibits a strict substrate specificity towards its biosynthetically proper substrate, delphinidin 3-(4-coumaroyl)-rutinoside | Petunia x hybrida Surfinia Purple | UDP + delphinidin 3-O-(4-coumaroyl)-rutinoside 5-O-beta-D-glucoside | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52163, PH1, sequence calculation | Petunia x hybrida |
Synonyms | Comment | Organism |
---|---|---|
5-GT | - |
Petunia x hybrida |
petunia 5-GT | - |
Petunia x hybrida |
PH1 | - |
Petunia x hybrida |
UDP-glucose:anthocyanin 5-O-glucosyltransferase | - |
Petunia x hybrida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Petunia x hybrida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Petunia x hybrida |
General Information | Comment | Organism |
---|---|---|
evolution | PH1 belongs to the 5-GT subfamily of the superfamily of glycosyltransferases | Petunia x hybrida |
physiological function | glucosylation at the 5-O-position of anthocyanins, which is catalyzed by anthocyanin 5-O-glucosyltransferase, is an important step to produce stable complexes in co-pigmentation, resulting in a bright reddish-purple color from dull violet or pure red | Petunia x hybrida |