Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | Campylobacter jejuni | - |
dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | Campylobacter jejuni NCTC 11168 | - |
dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
gene pglA, cj1125c | - |
Campylobacter jejuni NCTC 11168 | - |
gene pglA, cj1125c | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
Campylobacter jejuni | dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
Campylobacter jejuni NCTC 11168 | dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
additional information | in vitro transfer of N,N'-diacetylbacillosamine phosphate onto Und-P and the in vitro enzymatic synthesis of the heptasacccharide using a chemically synthesized undecaprenyl diphosphate-linked N,N'-diacetylbacillosamine. The reactions of PglC, PglA, PglH, PglJ and PglI are coupled in a single reaction vessel, NMR spectrometric product analysis | Campylobacter jejuni | ? | - |
? | |
additional information | in vitro transfer of N,N'-diacetylbacillosamine phosphate onto Und-P and the in vitro enzymatic synthesis of the heptasacccharide using a chemically synthesized undecaprenyl diphosphate-linked N,N'-diacetylbacillosamine. The reactions of PglC, PglA, PglH, PglJ and PglI are coupled in a single reaction vessel, NMR spectrometric product analysis | Campylobacter jejuni NCTC 11168 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cj1125c | - |
Campylobacter jejuni |
PglA | - |
Campylobacter jejuni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Campylobacter jejuni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Campylobacter jejuni |
General Information | Comment | Organism |
---|---|---|
metabolism | the Pgl pathway produces the N-linked glycan heptasaccharide GalNAc-alpha1,4-GalNAc-alpha1,4-(Glcbeta1,3)-GalNAc-alpha1,4-GalNAc-alpha1,4-GalNAc-alpha1,3-Bac2,4diNAc-_1-Asn. The pathway begins with diphosphate bond formation between the Bac2,4diNAc phosphate and undecaprenylphosphate (Und-P) by Cj1124c (PglC) to form Bac2,4diNAc-alpha1-PP-Und. One N-acetylgalactosamine (GalNAc) is linked to the polyisoprenediphosphate-bound N,N'-diacetylbacillosamine by Cj1125c (PglA) to form GalNAc-alpha1,3-Bac2,4diNAc-alpha1-PP-Und. Next, four additional GalNAc and one branching glucose are added sequentially to the isoprene-linked disaccharide by PglJ, PglH, and PglI, to form the heptasaccharide, overview | Campylobacter jejuni |