Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by UDP-2''-O-methyl-alpha-D-galactofuranose | Mycobacterium tuberculosis | |
UDP-3''-deoxy-alpha-D-xylo-hexofuranose | 68% inhibition at 1.25 mM | Mycobacterium tuberculosis | |
UDP-5''-O-methyl-alpha-D-galactofuranose | 15% inhibition at 1.25 mM | Mycobacterium tuberculosis | |
UDP-5-deoxy-alpha-D-galactofuranose | 10% inhibition at 1.25 mM | Mycobacterium tuberculosis | |
UDP-6''-O-methyl-alpha-D-galactofuranose | 26% inhibition at 1.25 mM | Mycobacterium tuberculosis | |
UDP-alpha-D-arabinofuranose | less than 1% inhibition at 1.25 mM | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
UDP-alpha-D-galactofuranose | using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis | |
0.5 | - |
UDP-3-deoxy-alpha-D-threo-hexofuranose | using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis | |
0.9 | - |
UDP-5-deoxy-alpha-D-galactofuranose | using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis | |
0.9 | - |
UDP-alpha-D-arabinofuranose | using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis | |
1 | - |
UDP-6-deoxy-alpha-D-galactofuranose | using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis | |
1 | - |
UDP-alpha-D-galactofuranose | using beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis | |
1.9 | - |
dTDP-alpha-D-galactofuranose | using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0008 | - |
using UDP-5-deoxy-alpha-D-galactofuranose and beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis |
0.004 | - |
using UDP-2''-O-methyl-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis |
0.006 | - |
using UDP-5''-O-methyl-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis |
0.007 | - |
using UDP-6''-O-methyl-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis |
0.01 | - |
using UDP-3-deoxy-alpha-D-threo-hexofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis |
0.01 | - |
using UDP-alpha-D-arabinofuranose and beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis |
0.012 | - |
using UDP-6-deoxy-alpha-D-galactofuranose and beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis |
0.1 | - |
using UDP-5-deoxy-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis |
0.14 | - |
using UDP-alpha-D-arabinofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis |
0.14 | - |
using UDP-alpha-D-galactofuranose and beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis |
0.35 | - |
using dTDP-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis |
0.9 | - |
using UDP-6-deoxy-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis |
1.6 | - |
using UDP-alpha-D-galactofuranose and beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as substrates, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl | - |
Mycobacterium tuberculosis | UDP + ? | - |
? | |
UDP-2''-O-methyl-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl | - |
Mycobacterium tuberculosis | UDP + ? | - |
? | |
UDP-3''-deoxy-alpha-D-xylo-hexofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl | - |
Mycobacterium tuberculosis | UDP + ? | - |
? | |
UDP-3-deoxy-alpha-D-threo-hexofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl | - |
Mycobacterium tuberculosis | UDP + ? | - |
? | |
UDP-5''-O-methyl-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl | - |
Mycobacterium tuberculosis | UDP + ? | - |
? | |
UDP-5-deoxy-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl | - |
Mycobacterium tuberculosis | UDP + ? | - |
? | |
UDP-5-deoxy-alpha-D-galactofuranose + beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl | - |
Mycobacterium tuberculosis | UDP + ? | - |
? | |
UDP-6''-O-methyl-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl | - |
Mycobacterium tuberculosis | UDP + ? | - |
? | |
UDP-6-deoxy-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl | - |
Mycobacterium tuberculosis | UDP + ? | - |
? | |
UDP-6-deoxy-alpha-D-galactofuranose + beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl | - |
Mycobacterium tuberculosis | UDP + ? | - |
? | |
UDP-alpha-D-arabinofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl | - |
Mycobacterium tuberculosis | UDP + ? | - |
? | |
UDP-alpha-D-arabinofuranose + beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl | - |
Mycobacterium tuberculosis | UDP + ? | - |
? | |
UDP-alpha-D-galactofuranose + beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl | best donor-acceptor combination, dual recognition of both the terminal C-5 and C-6 hydroxyl groups of the acceptor substrate are required for GlfT2 activity | Mycobacterium tuberculosis | UDP + ? | - |
? | |
UDP-alpha-D-galactofuranose + beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl | - |
Mycobacterium tuberculosis | UDP + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GlfT2 | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
UDP-3-deoxy-alpha-D-threo-hexofuranose | using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis | |
0.17 | - |
UDP-5-deoxy-alpha-D-galactofuranose | using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis | |
0.25 | - |
UDP-alpha-D-arabinofuranose | using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis | |
0.33 | - |
UDP-alpha-D-galactofuranose | using beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis | |
1.32 | - |
UDP-6-deoxy-alpha-D-galactofuranose | using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis | |
1.62 | - |
dTDP-alpha-D-galactofuranose | using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis | |
2.18 | - |
UDP-alpha-D-galactofuranose | using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
UDP-3''-deoxy-alpha-D-xylo-hexofuranose | in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
UDP-3-deoxy-alpha-D-threo-hexofuranose | using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis | |
0.2 | - |
UDP-5-deoxy-alpha-D-galactofuranose | using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis | |
0.25 | - |
UDP-alpha-D-arabinofuranose | using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis | |
0.33 | - |
dTDP-alpha-D-galactofuranose | using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis | |
0.33 | - |
UDP-alpha-D-galactofuranose | using beta-Galf-(1->6)-beta-Galf-(1->5)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis | |
1.17 | - |
UDP-6-deoxy-alpha-D-galactofuranose | using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis | |
8.3 | - |
UDP-alpha-D-galactofuranose | using beta-Galf-(1->5)-beta-Galf-(1->6)-beta-Galf-octyl as cosubstrate, in 50 mM MOPS pH 7.6 with 20 mM MgCl2, 5 mM 2-mercaptoethanol, at 37°C | Mycobacterium tuberculosis |