Cloned (Comment) | Organism |
---|---|
expressed as a His-tagged fusion protein in Escherichia coli | Streptomyces fradiae |
expressed in Escherichia coli BL21(DE3) cells | Streptomyces sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 55% residual activity in the presence of 2 mM | Streptomyces sp. | |
Co2+ | about 90% residual activity in the presence of 2 mM | Streptomyces sp. | |
Cu2+ | inhibits activity | Streptomyces fradiae | |
Cu2+ | complete inhibition in the presence of 2 mM | Streptomyces sp. | |
EDTA | about 20% residual activity in the presence of 10 mM | Streptomyces sp. | |
Ni2+ | about 80% residual activity in the presence of 2 mM | Streptomyces sp. | |
Zn2+ | inhibits activity | Streptomyces fradiae | |
Zn2+ | complete inhibition in the presence of 2 mM | Streptomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Streptomyces fradiae | |
Mg2+ | 100% activity in the presence of 2 mM | Streptomyces sp. | |
Mn2+ | - |
Streptomyces fradiae | |
Mn2+ | 105% activity in the presence of 2 mM Mn2+ compared to Mg2+ | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces fradiae | - |
- |
- |
Streptomyces sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE Sepharose column chromatography, Superdex Hi-Load 200 gel filtration, and Mono Q column chromatography | Streptomyces sp. |
purified to homogeneity by DEAE Sepharose column chromatography, gel filtration, and MonoQ column chromatography | Streptomyces fradiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + ribostamycin | - |
Streptomyces sp. | UDP + 2'''-acetyl-6'''-hydroxyneomycin C | - |
? | |
UDP-N-acetyl-alpha-D-glucosamine + ribostamycin | - |
Streptomyces fradiae | UDP + 2'''-acetyl-6'''-hydroxyneomycin C | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Neo15 | - |
Streptomyces sp. |
neoK | - |
Streptomyces sp. |
neoK | - |
Streptomyces fradiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Streptomyces fradiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Streptomyces fradiae |
General Information | Comment | Organism |
---|---|---|
metabolism | NeoK is a glycosyltransferase that catalyzes the retaining glycosylation of ribostamycin with UDP-GlcNAc as a sugar donor | Streptomyces fradiae |