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Literature summary for 2.4.1.285 extracted from

  • Yokoyama, K.; Yamamoto, Y.; Kudo, F.; Eguchi, T.
    Involvement of two distinct N-acetylglucosaminyltransferases and a dual-function deacetylase in neomycin biosynthesis (2008), ChemBioChem, 9, 865-869.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a His-tagged fusion protein in Escherichia coli Streptomyces fradiae
expressed in Escherichia coli BL21(DE3) cells Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ about 55% residual activity in the presence of 2 mM Streptomyces sp.
Co2+ about 90% residual activity in the presence of 2 mM Streptomyces sp.
Cu2+ inhibits activity Streptomyces fradiae
Cu2+ complete inhibition in the presence of 2 mM Streptomyces sp.
EDTA about 20% residual activity in the presence of 10 mM Streptomyces sp.
Ni2+ about 80% residual activity in the presence of 2 mM Streptomyces sp.
Zn2+ inhibits activity Streptomyces fradiae
Zn2+ complete inhibition in the presence of 2 mM Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Streptomyces fradiae
Mg2+ 100% activity in the presence of 2 mM Streptomyces sp.
Mn2+
-
Streptomyces fradiae
Mn2+ 105% activity in the presence of 2 mM Mn2+ compared to Mg2+ Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces fradiae
-
-
-
Streptomyces sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Sepharose column chromatography, Superdex Hi-Load 200 gel filtration, and Mono Q column chromatography Streptomyces sp.
purified to homogeneity by DEAE Sepharose column chromatography, gel filtration, and MonoQ column chromatography Streptomyces fradiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + ribostamycin
-
Streptomyces sp. UDP + 2'''-acetyl-6'''-hydroxyneomycin C
-
?
UDP-N-acetyl-alpha-D-glucosamine + ribostamycin
-
Streptomyces fradiae UDP + 2'''-acetyl-6'''-hydroxyneomycin C
-
?

Synonyms

Synonyms Comment Organism
Neo15
-
Streptomyces sp.
neoK
-
Streptomyces sp.
neoK
-
Streptomyces fradiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Streptomyces fradiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Streptomyces fradiae

General Information

General Information Comment Organism
metabolism NeoK is a glycosyltransferase that catalyzes the retaining glycosylation of ribostamycin with UDP-GlcNAc as a sugar donor Streptomyces fradiae