Cloned (Comment) | Organism |
---|---|
co-expressed with molecular chaperone GroES and GroEL in Escherichia coli BL21(DE3) cells | Streptomyces sp. |
expressed as a His-tagged fusion protein in Escherichia coli | Streptomyces fradiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces fradiae | - |
- |
- |
Streptomyces sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
a poor amount is isolated using Ni-NTA chromatography. The cell-free extract of Escherichia coli expressing NeoD is used for enzymatic assays | Streptomyces fradiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | other possible glycosyl donor, UDP-glucose, and other possible glycosyl acceptors, paromamine, neamine, and ribostamycin are not accepted | Streptomyces fradiae | ? | - |
? | |
additional information | the enzyme is unable to catalyze the glycosylation of ribostamycin | Streptomyces sp. | ? | - |
? | |
UDP-N-acetyl-alpha-D-glucosamine + 2-deoxystreptamine | - |
Streptomyces fradiae | UDP + 2'-N-acetylparomamine | - |
? | |
UDP-N-acetyl-alpha-D-glucosamine + 2-deoxystreptamine | about 80% of 2-deoxystreptamine is conversed | Streptomyces sp. | UDP + 2'-N-acetylparomamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
neoD | - |
Streptomyces sp. |
neoD | - |
Streptomyces fradiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Streptomyces fradiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Streptomyces fradiae |