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Literature summary for 2.4.1.281 extracted from

  • Ye, Y.; Saburi, W.; Odaka, R.; Kato, K.; Sakurai, N.; Komoda, K.; Nishimoto, M.; Kitaoka, M.; Mori, H.; Yao, M.
    Structural insights into the difference in substrate recognition of two mannoside phosphorylases from two GH130 subfamilies (2016), FEBS Lett., 590, 828-837.
    View publication on PubMed
Search for more literature for 2.4.1.281

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure with/without 4-O-beta-D-mannosyl-D-glucose. His245 of loop 3 forms a hydrogen-bond network with the substrate through a water molecule, and is indispensible for substrate binding Ruminococcus albus

Protein Variants

Protein Variants Comment Organism
H245A 1.47% of wild-type phosphorolytic activity Ruminococcus albus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.41
-
 alpha-D-mannose 1-phosphate wild-type, pH 6.5, 37°C Ruminococcus albus
25.9
-
 D-glucose wild-type, pH 6.5, 37°C Ruminococcus albus
36.5
-
 alpha-D-mannose 1-phosphate mutant H245A, pH 6.5, 37°C Ruminococcus albus
319
-
 D-glucose mutant H245A, pH 6.5, 37°C Ruminococcus albus

Organism

Organism UniProt Comment Textmining
Ruminococcus albus E6UIS7
-
-
Ruminococcus albus DSM 20455 E6UIS7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
-
 ?
D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus DSM 20455 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
MP1
-
 Ruminococcus albus
Rumal_0852
-
 Ruminococcus albus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.76
-
 alpha-D-mannose 1-phosphate mutant H245A, pH 6.5, 37°C Ruminococcus albus
19.3
-
 D-glucose mutant H245A, pH 6.5, 37°C Ruminococcus albus
52.1
-
 alpha-D-mannose 1-phosphate wild-type, pH 6.5, 37°C Ruminococcus albus
126
-
 D-glucose wild-type, pH 6.5, 37°C Ruminococcus albus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.061
-
 D-glucose mutant H245A, pH 6.5, 37°C Ruminococcus albus
0.076
-
 alpha-D-mannose 1-phosphate mutant H245A, pH 6.5, 37°C Ruminococcus albus
4.86
-
 D-glucose wild-type, pH 6.5, 37°C Ruminococcus albus
21.6
-
 alpha-D-mannose 1-phosphate wild-type, pH 6.5, 37°C Ruminococcus albus