Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 | Ruminococcus albus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-D-mannose 1-phosphate | competitive; competitive | Ruminococcus albus | |
cellobiose | - |
Ruminococcus albus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.304 | - |
4-O-beta-D-mannopyranosyl-D-glucopyranose | pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate | Ruminococcus albus | |
0.324 | - |
alpha-D-mannose 1-phosphate | pH 6.5, 37°C, synthetic reaction, cosubstrate D-glucose | Ruminococcus albus | |
0.444 | - |
phosphate | pH 6.5, 37°C, phosphorolytic reaction, cosubstrate 4-O-beta-D-mannopyranosyl-D-glucopyranose | Ruminococcus albus | |
5.34 | - |
phosphate | pH 6.5, 37°C, phosphorolytic reaction, cosubstrate 4-O-beta-D-mannopyranosyl-D-glucopyranose | Ruminococcus albus | |
5.8 | - |
alpha-D-mannose 1-phosphate | pH 6.5, 37°C, synthetic reaction, cosubstrate D-glucose | Ruminococcus albus | |
6.14 | - |
D-allose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
6.44 | - |
3-deoxy-3-fluoro-D-glucose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
7.82 | - |
3-O-methyl-D-glucose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
22.2 | - |
cellobiose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
27.7 | - |
4-O-beta-D-mannopyranosyl-D-glucopyranose | pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate | Ruminococcus albus | |
29.1 | - |
laminaribiose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
38.2 | - |
D-glucose | pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
45.6 | - |
D-mannose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
48.7 | - |
2-deoxy-2-fluoro-D-glucose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
49.8 | - |
sophorose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
49.9 | - |
N,N'-diacetylchitobiose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
50.4 | - |
maltose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
60.8 | - |
gentiobiose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
79.2 | - |
1,5-anhydro-D-glucitol | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
85.2 | - |
methyl beta-D-glucoside | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
85.3 | - |
methyl alpha-D-glucoside | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
90.7 | - |
2-deoxy-D-glucose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
107 | - |
N-acetyl-D-glucosamine | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
119 | - |
6-deoxy-D-glucose | pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
156 | - |
6-deoxy-6-fluoro-D-glucose | pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
172 | - |
D-glucose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Ruminococcus albus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37823 | - |
6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence | Ruminococcus albus |
38000 | - |
6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence | Ruminococcus albus |
43646 | - |
2 * 45000, SDS-PAGE, 4 * 43646, amino acid sequence | Ruminococcus albus |
45000 | - |
2 * 45000, SDS-PAGE, 4 * 43646, amino acid sequence | Ruminococcus albus |
80000 | - |
gel filtration | Ruminococcus albus |
209000 | - |
gel filtration | Ruminococcus albus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate | Ruminococcus albus | - |
D-glucose + alpha-D-mannose 1-phosphate | - |
r | |
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate | Ruminococcus albus NE1 | - |
D-glucose + alpha-D-mannose 1-phosphate | - |
r | |
6-deoxy-6-fluoro-D-glucose + alpha-D-mannose 1-phosphate | Ruminococcus albus | - |
4-O-beta-D-mannopyranosyl-6-deoxy-6-fluoro-D-glucopyranose + phosphate | - |
? | |
6-deoxy-6-fluoro-D-glucose + alpha-D-mannose 1-phosphate | Ruminococcus albus NE1 | - |
4-O-beta-D-mannopyranosyl-6-deoxy-6-fluoro-D-glucopyranose + phosphate | - |
? | |
6-deoxy-D-glucose + alpha-D-mannose 1-phosphate | Ruminococcus albus | - |
4-O-beta-D-mannopyranosyl-6-deoxy-D-glucopyranose + phosphate | - |
? | |
6-deoxy-D-glucose + alpha-D-mannose 1-phosphate | Ruminococcus albus NE1 | - |
4-O-beta-D-mannopyranosyl-6-deoxy-D-glucopyranose + phosphate | - |
? | |
D-glucose + alpha-D-mannose 1-phosphate | Ruminococcus albus | - |
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate | - |
r | |
D-glucose + alpha-D-mannose 1-phosphate | Ruminococcus albus NE1 | - |
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate | - |
r | |
laminaribiose + alpha-D-mannose 1-phosphate | Ruminococcus albus | - |
? + phosphate | - |
? | |
laminaribiose + alpha-D-mannose 1-phosphate | Ruminococcus albus NE1 | - |
? + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ruminococcus albus | E6UBR9 | - |
- |
Ruminococcus albus | E6UIS7 | - |
- |
Ruminococcus albus NE1 | E6UBR9 | - |
- |
Ruminococcus albus NE1 | E6UIS7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Ruminococcus albus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate = D-glucose + alpha-D-mannose 1-phosphate | sequential Bi Bi mechanism | Ruminococcus albus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-anhydro-D-glucitol + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | 4-O-beta-D-mannopyranosyl-1,5-anhydro-D-glucitol + phosphate | - |
r | |
1,5-anhydro-D-glucitol + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus NE1 | 4-O-beta-D-mannopyranosyl-1,5-anhydro-D-glucitol + phosphate | - |
r | |
2-deoxy-2-fluoro-D-glucose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | 4-O-beta-D-mannopyranosyl-2-deoxy-2-fluoro-D-glucopyranose + phosphate | - |
r | |
2-deoxy-D-glucose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | 4-O-beta-D-mannopyranosyl-2-deoxy-D-glucopyranose + phosphate | - |
r | |
3-deoxy-3-fluoro-D-glucose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | 4-O-beta-D-mannopyranosyl-3-deoxy-3-fluoro-D-glucopyranose + phosphate | - |
r | |
3-O-methyl-D-glucose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | 4-O-beta-D-mannopyranosyl-3-O-methyl-D-glucopyranose + phosphate | - |
r | |
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate | - |
Ruminococcus albus | D-glucose + alpha-D-mannose 1-phosphate | - |
r | |
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate | - |
Ruminococcus albus NE1 | D-glucose + alpha-D-mannose 1-phosphate | - |
r | |
6-deoxy-6-fluoro-D-glucose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | 4-O-beta-D-mannopyranosyl-6-deoxy-6-fluoro-D-glucopyranose + phosphate | - |
? | |
6-deoxy-6-fluoro-D-glucose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus NE1 | 4-O-beta-D-mannopyranosyl-6-deoxy-6-fluoro-D-glucopyranose + phosphate | - |
? | |
6-deoxy-D-glucose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | 4-O-beta-D-mannopyranosyl-6-deoxy-D-glucopyranose + phosphate | - |
? | |
6-deoxy-D-glucose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus NE1 | 4-O-beta-D-mannopyranosyl-6-deoxy-D-glucopyranose + phosphate | - |
? | |
cellobiose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | ? + phosphate | - |
r | |
D-allose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | 4-O-beta-D-mannopyranosyl-D-allose + phosphate | - |
r | |
D-glucose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate | - |
r | |
D-glucose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus NE1 | 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate | - |
r | |
D-mannose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | 4-O-beta-D-mannopyranosyl-D-mannopyranose + phosphate | - |
r | |
gentiobiose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | ? + phosphate | - |
r | |
laminaribiose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | ? + phosphate | - |
r | |
laminaribiose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | ? + phosphate | - |
? | |
laminaribiose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus NE1 | ? + phosphate | - |
r | |
laminaribiose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus NE1 | ? + phosphate | - |
? | |
maltose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | ? + phosphate | - |
r | |
methyl alpha-D-glucoside + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | methyl [4-O-beta-D-mannopyranosyl]-alpha-D-glucoside + phosphate | - |
r | |
methyl beta-D-glucoside + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | methyl [4-O-beta-D-mannopyranosyl]-beta-D-glucopyranoside + phosphate | - |
r | |
additional information | The enzyme also catalyzes the reaction of beta-1,4-mannooligosaccharide phosphorylase | Ruminococcus albus | ? | - |
? | |
additional information | The enzyme also catalyzes the reaction of beta-1,4-mannooligosaccharide phosphorylase | Ruminococcus albus NE1 | ? | - |
? | |
N,N'-diacetylchitobiose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | ? + phosphate | - |
r | |
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate | - |
r | |
sophorose + alpha-D-mannose 1-phosphate | - |
Ruminococcus albus | ? + phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 45000, SDS-PAGE, 4 * 43646, amino acid sequence | Ruminococcus albus |
homohexamer | 6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence | Ruminococcus albus |
Synonyms | Comment | Organism |
---|---|---|
RaMp1 | - |
Ruminococcus albus |
RaMp2 | - |
Ruminococcus albus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Ruminococcus albus |
50 | - |
- |
Ruminococcus albus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to | Ruminococcus albus |
45 | - |
stable up to | Ruminococcus albus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.667 | - |
D-allose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
0.763 | - |
3-O-methyl-D-glucose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
0.94 | - |
3-deoxy-3-fluoro-D-glucose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
5.07 | - |
maltose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
5.12 | - |
D-mannose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
5.71 | - |
2-deoxy-2-fluoro-D-glucose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
6.34 | - |
N,N'-diacetylchitobiose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
11.6 | - |
laminaribiose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
11.7 | - |
2-deoxy-D-glucose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
13.8 | - |
sophorose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
15.8 | - |
N-acetyl-D-glucosamine | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
17.5 | - |
4-O-beta-D-mannopyranosyl-D-glucopyranose | pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate | Ruminococcus albus | |
20.2 | - |
cellobiose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
20.7 | - |
1,5-anhydro-D-glucitol | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
20.7 | - |
methyl alpha-D-glucoside | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
26.1 | - |
gentiobiose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
34.5 | - |
D-glucose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
35.2 | - |
methyl beta-D-glucoside | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
42 | - |
6-deoxy-6-fluoro-D-glucose | pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
45.1 | - |
D-glucose | pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
83.3 | - |
4-O-beta-D-mannopyranosyl-D-glucopyranose | pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate | Ruminococcus albus | |
177 | - |
6-deoxy-D-glucose | pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Ruminococcus albus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 9.5 | 4°C, 24 h stable | Ruminococcus albus |
4.5 | 10.5 | 4°C, 24 h stable | Ruminococcus albus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0976 | - |
3-O-methyl-D-glucose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
0.101 | - |
maltose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
0.109 | - |
D-allose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
0.112 | - |
D-mannose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
0.117 | - |
2-deoxy-2-fluoro-D-glucose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
0.127 | - |
N,N'-diacetylchitobiose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
0.129 | - |
2-deoxy-D-glucose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
0.146 | - |
3-deoxy-3-fluoro-D-glucose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
0.148 | - |
N-acetyl-D-glucosamine | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
0.201 | - |
D-glucose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
0.236 | - |
methyl alpha-D-glucoside | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
0.261 | - |
1,5-anhydro-D-glucitol | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
0.269 | - |
6-deoxy-6-fluoro-D-glucose | pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
0.277 | - |
sophorose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
0.399 | - |
laminaribiose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
0.413 | - |
methyl beta-D-glucoside | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
0.429 | - |
gentiobiose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
0.91 | - |
cellobiose | apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus | |
1.49 | - |
6-deoxy-D-glucose | pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate | Ruminococcus albus |