Application | Comment | Organism |
---|---|---|
medicine | the activated enzyme is capable of transferring an alternative unnatural sugar donor. Because the desosamine sugar contacts the 50S subunit of ribosome, the discovery that an unnatural sugar can be transferred has implications for the synthesis of new erythromycin derivatives with improved properties. Reconstitute of the activity of EryCIII in vitro for the synthesis of unnatural macrolides | Saccharopolyspora erythraea |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) as a C-terminal His6 fusion protein | Saccharopolyspora erythraea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
3-alpha-mycarosylerythronolide B | pH and temperature not specified in the publication, 0.05 mM dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose | Saccharopolyspora erythraea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 3-alpha-mycarosylerythronolide B | Saccharopolyspora erythraea | dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose i.e. dTDP-D-desosamine | dTDP + erythromycin D | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharopolyspora erythraea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharopolyspora erythraea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 3-alpha-mycarosylerythronolide B | dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose i.e. dTDP-D-desosamine | Saccharopolyspora erythraea | dTDP + erythromycin D | - |
? | |
dTDP-D-mycaminose + 3-alpha-mycarosylerythronolide B | the activated enzyme is capable of transferring an alternative sugar donor | Saccharopolyspora erythraea | dTDP + erythromycin M | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EryCIII | - |
Saccharopolyspora erythraea |
glycosyltransferase EryCIII | - |
Saccharopolyspora erythraea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
3-alpha-mycarosylerythronolide B | pH and temperature not specified in the publication, 0.05 mM TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose | Saccharopolyspora erythraea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
AKnT protein | the noncognate glycosyltransferase activator protein AknT, stably activates EryCIII. Addition of purified AknT to inactive EryCIII restores the activity of EryCIII in vitro | Saccharopolyspora erythraea | |
EryCII protein | glycosyltransferase activator protein EryCII activates EryCIII. EryCIII treated with EryCII is catalytically active, effecting transfer of desosamine to 3-alpha-mycarosylerythronolide. The untreated EryCIII sample shows no activity | Saccharopolyspora erythraea |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in erythromycin biosynthesis | Saccharopolyspora erythraea |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
3-alpha-mycarosylerythronolide B | pH and temperature not specified in the publication, 0.05 mM dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose | Saccharopolyspora erythraea |