Application | Comment | Organism |
---|---|---|
medicine | potential use to make unnatural macrolides for use as antibacterial | Saccharopolyspora erythraea |
Cloned (Comment) | Organism |
---|---|
coexpression of EryCIII with the GroEL/ES chaperone complex greatly enhances the expression of soluble EryCIII protein | Saccharopolyspora erythraea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharopolyspora erythraea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharopolyspora erythraea |
Storage Stability | Organism |
---|---|
4°C, activity of concentrated enzyme stored overnight is more than 3 times lower than that of freshly purified enzyme. | Saccharopolyspora erythraea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 3-alpha-mycarosylerythronolide B | TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose i.e. TDP-D-desosamine | Saccharopolyspora erythraea | dTDP + erythromycin D | product detected by HPLC. Identity is conformed via mass spectrometry and chromatographic comparison with an authentic reference standard | ? | |
additional information | no activity with: TDP-D-mycaminose, 6-deoxy-erythronolide B, 10-deoxymethynolide | Saccharopolyspora erythraea | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EryCIII | - |
Saccharopolyspora erythraea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Saccharopolyspora erythraea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
3-alpha-mycarosylerythronolide B | pH 8.0, 25°C, in the presence og 0.001 mM EryC and 1 mM of each substrate, more than 300 turnovers are observed in 2.5 min, indicating that the kcat must be greater than 100/min. This is one of the highest turnover numbers reported for an antibiotic glycosyl transferase | Saccharopolyspora erythraea | |
1.7 | - |
dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose | pH 8.0, 25°C, in the presence og 0.001 mM EryC and 1 mM of each substrate, more than 300 turnovers are observed in 2.5 min, indicating that the kcat must be greater than 100/min. This is one of the highest turnover numbers reported for an antibiotic glycosyl transferase | Saccharopolyspora erythraea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Saccharopolyspora erythraea |