Literature summary for 2.4.1.272 extracted from

Shibuya, M.; Nishimura, K.; Yasuyama, N.; Ebizuka, Y.
Identification and characterization of glycosyltransferases involved in the biosynthesis of soyasaponin I in Glycine max (2010), FEBS Lett., 584, 2258-2264.

Search for more literature for 2.4.1.272

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Glycine max

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.012	-	soyasapogenol B 3-O-beta-D-glucuronide	pH 7.4, 37°C	Glycine max

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55866	-	x * 55866, calculated from sequence	Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-galactose + soyasapogenol B 3-O-beta-D-glucuronide	Glycine max	the enzyme is involved in the biosynthesis of soyasaponin I in Glycine max	UDP + soyasaponin III	-	?

Organism

Organism	UniProt	Comment	Textmining
Glycine max	D4Q9Z4	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-galactose + soyasapogenol B 3-O-beta-D-glucuronide	the enzyme is involved in the biosynthesis of soyasaponin I in Glycine max	Glycine max	UDP + soyasaponin III	-	?
UDP-galactose + soyasapogenol B 3-O-beta-D-glucuronide	high substrate specificity for soyasapogenol B 3-O-beta-D-glucuronide	Glycine max	UDP + soyasaponin III	soyasaponin III i.e. 3beta-(2-O-beta-D-galactopyranosyl-beta-D-glucopyranosyloxyuronic acid)olean-12-ene-22beta,24-diol	?

Subunits

Subunits	Comment	Organism
?	x * 55866, calculated from sequence	Glycine max

Synonyms

Synonyms	Comment	Organism
GmSGT2	-	Glycine max
UDP-galactose:SBMG-galactosyltransferase	-	Glycine max
UGT73P2	-	Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Glycine max

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Glycine max

General Information

General Information	Comment	Organism
physiological function	part of the biosynthetic pathway for soyasaponins	Glycine max

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Release 2023.1 (January 2023)