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Literature summary for 2.4.1.262 extracted from

  • Kurosawa, Y.; Takahara, H.; Shiraiwa, M.
    UDP-glucuronic acid:soyasapogenol glucuronosyltransferase involved in saponin biosynthesis in germinating soybean seeds (2002), Planta, 215, 620-629.
    View publication on PubMed
Search for more literature for 2.4.1.262

General Stability

General Stability Organism
CHAPS > deoxy-BIGCHAP > n-octyl-beta-D-thioglucoside. Sodium cholate, SM-1200, and n-dodecyl-beta-D-maltoside do not effectively solubilize UGASGT Glycine max
the effectiveness of detergents at the CMC in solubilizing UGASGT is as follows: MEGA10 > SM-1000 > Glycine max

Inhibitors

Inhibitors Comment Organism Structure
Mega-10 the detergent inactivates UGASGT during solubilization Glycine max
SM-1000 the detergent inactivates UGASGT during solubilization Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00403
-
 soyasapogenol A in 100 mM Tris-HCl (pH 8.0), at 40°C Glycine max
0.01722
-
 soyasapogenol B in 100 mM Tris-HCl (pH 8.0), at 40°C Glycine max
0.02394
-
 UDP-D-glucuronate using soyasapogenol B as the sugar acceptor, in 100 mM Tris-HCl (pH 8.0), at 40°C Glycine max
0.04194
-
 soyasapogenol E in 100 mM Tris-HCl (pH 8.0), at 40°C Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Glycine max
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the effectiveness of divalent cations at 10 mM in activating UGASGT is as follows: Mg2+>Mn2+>Ca2+ Glycine max
Mg2+ the optimum concentration of Mg2+ is between 10 and 25 mM. UGASGT shows an absolute requirement for divalent cations. The effectiveness of divalent cations at 10 mM in activating UGASGT is as follows: Mg2+>Mn2+>Ca2+ Glycine max
Mn2+ the effectiveness of divalent cations at 10 mM in activating UGASGT is as follows: Mg2+>Mn2+>Ca2+ Glycine max
additional information Na+, K+, Co2+, Fe2+, Zn2+, Cu2+, Al3+, and Fe3+, activate UGASGT only slightly or not at all Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 the 66000 Da and/or the 50000 Da proteins are the UGASGT protein as determined by SDS-PAGE Glycine max
66000
-
 the 66000 Da and/or the 50000 Da proteins are the UGASGT protein as determined by SDS-PAGE Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Q column chromatography, Superdex 200 gel filtration, and HiTrap Blue column chromatography column chromatography Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.342
-
 crude extract, in 100 mM Tris-HCl (pH 8.0), at 40°C Glycine max
7
-
 after 204.68fold purification, in 100 mM Tris-HCl (pH 8.0), at 40°C Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information beta-amyrin, sophoradiol, and glycyrrhetinic acid are not converted by UGASGT. UGASGT does not catalyze the transfer reaction of glucuronate to soyasapogenol B-3-O-beta-glucuronide Glycine max ?
-
 ?
UDP-D-glucuronate + soyasapogenol A 40.6% activity compared to soyasapogenol B Glycine max UDP + soyasapogenol A 3-O-D-glucuronide
-
 ?
UDP-D-glucuronate + soyasapogenol B soyasapogenol B is the best sugar acceptor, 100% activity Glycine max UDP + soyasapogenol B 3-O-D-glucuronide
-
 ?
UDP-D-glucuronate + soyasapogenol E 79.08% activity compared to soyasapogenol B Glycine max UDP + soyasapogenol E 3-O-D-glucuronide
-
 ?
UDP-galactose + soyasapogenol B 8.35% activity compared to UDP-D-glucuronate Glycine max ?
-
 ?
UDP-glucose + soyasapogenol B 7.94% activity compared to UDP-D_glucuronate Glycine max ?
-
 ?

Synonyms

Synonyms Comment Organism
UDP-glucuronic acid:soyasapogenol glucuronosyltransferase
-
 Glycine max
UGASGT
-
 Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 in Tris-HCl buffer (pH 8.0) Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
 Glycine max