Application | Comment | Organism |
---|---|---|
analysis | metabolic labeling method to introduce the diazirine photocross-linking functional group onto O-GlcNAc residues in mammalian cells. Cells are engineered to produce diazirine-modified UDP-GlcNAc (UDP-Glc-NDAz), which is transferred to substrate proteins by endogenous OGT, producing O-GlcNDAz. Modified proteins can be covalently cross-linked to their binding partners, providing information about O-GlcNAc-dependent interactions | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C917A | mutant efficiently transfers diazirine-modified GlcNDAz and has altered substrate specificity, preferring to transfer GlcNDAz rather than GlcNAc to protein substrates | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
UDP-GlcNAc | wild-type, pH 7.2 22°C | Homo sapiens | |
0.0047 | - |
UDP-N-azidoacetylglucosamine | wild-type, pH 7.2 22°C | Homo sapiens | |
0.0085 | - |
UDP-N-azidoacetylglucosamine | mutant C917A, pH 7.2 22°C | Homo sapiens | |
0.0155 | - |
UDP-GlcNAc | mutant C917A, pH 7.2 22°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein kinase II + UDP-GlcNAc | - |
Homo sapiens | ? + UDP | - |
? | |
casein kinase II + UDP-N-azidoacetylglucosamine | - |
Homo sapiens | ? + UDP | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
UDP-N-azidoacetylglucosamine | wild-type, pH 7.2 22°C | Homo sapiens | |
0.0027 | - |
UDP-GlcNAc | mutant C917A, pH 7.2 22°C | Homo sapiens | |
0.008 | - |
UDP-GlcNAc | wild-type, pH 7.2 22°C | Homo sapiens | |
0.0085 | - |
UDP-N-azidoacetylglucosamine | mutant C917A, pH 7.2 22°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
UDP-N-azidoacetylglucosamine | mutant C917A, pH 7.2 22°C | Homo sapiens | |
0.002 | - |
UDP-GlcNAc | mutant C917A, pH 7.2 22°C | Homo sapiens | |
0.003 | - |
UDP-N-azidoacetylglucosamine | wild-type, pH 7.2 22°C | Homo sapiens | |
0.0037 | - |
UDP-GlcNAc | wild-type, pH 7.2 22°C | Homo sapiens |